Proteomics Software | DIA Proteomics | Discovery Proteomics



The gold standard for DIA proteomics analysis

Thanks to deep-learning augmented peptide identification, you can now find more precursors and proteins in your samples than ever before. We have further broadened our support for the latest mass spectrometry data acquisition technologies, and provide new ways to quickly and thoroughly analyze data.

      Spectronaut® is a commercial software package aimed at analyzing data-independent acquisition (DIA) proteomics experiments. Spectronaut® can quantitatively profile 100s to several 1,000s of proteins in one experiment. Large experiments with several conditions and replicates consisting of up to 10,000s of LC-MS runs can be analyzed.


      1. Vendor Independent
      2. Usability
      3. Complete Workflow
      4. Performance and Scalability
      5. Versatility and Flexibility


      Check out what’s new in Spectronaut®17.

      Supported Instruments


      • Thermo Scientific™ Orbitrap Exploris™ 480
      • Thermo Scientific™ Q Exactive™ Series
      • Thermo Scientific™ Orbitrap Fusion™ Series
      • SCIEX ZenoTOF 7600 System
      • SCIEX TripleTOF® Series
      • Bruker Impact II
      • Bruker timsTOF™ and timsTOF™ Pro*
      • Waters Xevo® G2-XS
      Supported Methods


      • HRM™
      • dia-PASEF®
      • SWATH™
      • Zeno SWATH
      • BoxCar DIA
      • SONAR™
      • WiSIM-DIA


      Methods should acquire MS1 and MS2 or MS2 only scans. The cycle time of the method should be in the range of 1-3 seconds depending on your average peak width. Fractionation, including gas-phase fractionation, is supported.

      Recommended System Requirements*


      • Windows 10 x64 / Windows Server 2016
      • Intel or AMD CPU with 4 cores or more
      • 2 TB of free hard drive space or more (2x data set size)
      • 16 GB of memory or more (1 precursor in 1 run amounts to ~0.5 KB of RAM)


      *For a typical experiment of 200 runs with a 100k precursor library

      If you are interested in ordering one of our software, please submit a request form or contact us. Our team will review your inquiry and get back to you within 5 working days.

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        New: Spectronaut® 17

        A Groundbreaking Increase in Identifications

        New: Spectronaut® 17

        A Groundbreaking Increase in Identifications

        Learn More