Proteomics Software | Data-Independent Acquisition | DIA Proteomics

Software

Spectronaut®

The gold standard for DIA proteomics analysis

Thanks to deep-learning augmented peptide identification, you can now find more precursors and proteins in your samples than ever before. We have further broadened our support for the latest mass spectrometry data acquisition technologies, and provide new ways to quickly and thoroughly analyze data.

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      Spectronaut® is a commercial software package aimed at analyzing data-independent acquisition (DIA) proteomics experiments. Spectronaut® can quantitatively profile 100s to several 1,000s of proteins in one experiment. Large experiments with several conditions and replicates consisting of up to 10,000s of LC-MS runs can be analyzed.

      Features:

      1. Vendor Independent
      2. Usability
      3. Complete Workflow
      4. Performance and Scalability
      5. Versatility and Flexibility

       

      Check out what’s new in Spectronaut®19.

      Supported Instruments

       

      • Thermo Scientific™ Orbitrap Astral
      • Thermo Scientific™ Orbitrap Exploris™ 480
      • Thermo Scientific™ Q Exactive™ Series
      • Thermo Scientific™ Orbitrap Fusion™ Series
      • Bruker timsTOF™ Pro series and timsTOF HT
      • Bruker timsTOF™ SCP and timsTOF Ultra series
      • Bruker timsTOF™ Flex Bruker Impact II
      • SCIEX ZenoTOF 7600 System
      • SCIEX TripleTOF® Series
      • Waters Xevo® G2-XS
      Supported Methods

       

      • HRM™
      • dia-PASEF®
      • SWATH™
      • Zeno SWATH
      • BoxCar DIA
      • SONAR™
      • WiSIM-DIA

       

      Methods should acquire MS1 and MS2 or MS2 only scans. The cycle time of the method should be in the range of 1-3 seconds depending on your average peak width. Fractionation, including gas-phase fractionation, is supported.

      Recommended System Requirements*

       

      • Windows 10 x64 / Windows Server 2016 or later
      • Intel or AMD CPU with 4 cores or more
      • 2 TB of free hard drive space or more (2x data set size)
      • 16 GB of memory or more (1 precursor in 1 run amounts to ~0.5 KB of RAM)

       

      *for a library-free experiment with 200 DIA runs and typical search space settings

      If you are interested in ordering one of our software, please submit a request form or contact us. Our team will review your inquiry and get back to you within 5 working days.

      Filter By
      • About
        • Spectronaut software – aim and principles
        • What is Spectronaut?
          How does Spectronaut work?
          What is Pulsar?
        • Workflows available in the software
        • Can I perform library-free DIA analysis with Spectronaut? What is directDIA?
          Can I perform library-based DIA analysis in Spectronaut?
          Can I perform PTM analysis in Spectronaut?
          Does Spectronaut perform label-free quantification?
          Can I perform absolute protein quantification using Spectronaut?
      • Compatibility
        • Newest release - Spectronaut 19
        • What are the key features of the new Spectronaut release?
        • Supported data
        • What are the LC-MS instruments supported in Spectronaut?
          What are the acquisition methods supported in Spectronaut?
          Can I generate a library in Spectronaut based on the result from another search engine?
          Can I import an external spectral library to Spectronaut?
        • Workstation and setup for Spectronaut
        • What are the minimal and recommended system requirements for the software?
          What are system environments supported for Spectronaut?
          Does Spectronaut support multi-user configurations and analysis parallelization?
          Are Spectronaut licenses bound to a single system or floating?
      • Accessing Spectronaut
        • Getting the license
        • How can I get the Spectronaut license?
          What are Spectronaut licensing options?
        • Software trial
        • How can I get a Spectronaut trial?
          What is covered in the Spectronaut trial?
        • Viewer
        • How can I get Spectronaut Viewer?
          What features are available in Spectronaut Viewer?
      • Getting Help
        • Getting further help
        • Can I get a consultation on software applications and functions?
          How can I contact the support team about specific issues/questions I have about Spectronaut?
        • Introduction and tutorials to Spectronaut
        • How can I learn to use Spectronaut?
          Where can I find information about Spectronaut features?
          Where can I see any examples of Spectronaut being used?

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        New: Spectronaut® 19

        Unlock Your Data's True Story

        New: Spectronaut® 19

        Unlock Your Data's True Story

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