Spectronaut®
Commercial DIA software
Users trust Spectronaut
Papers cite Spectronaut
Years of software development
The gold standard for DIA proteomics analysis
Spectronaut is a high-performance DIA proteomics software solution engineered to deliver unparalleled throughput and precision. Its state-of-the-art signal processing and artificial intelligence (AI) augmentation ensure reproducible, high-accuracy quantification, while comprehensive visualization and flexible, customizable reporting facilitate in-depth data interrogation. Experience unmatched processing speed and technical rigor that empower your lab to push the boundaries of proteomics data analysis.
Specifications
Supported Instruments
Spectronaut supports DIA mass spectrometry data collection collected using instruments from Bruker, Thermo Scientific, SCIEX, and Waters.
Specific supported models include:
- Bruker timsTOF™ Pro series and timsTOF HT
- Bruker timsTOF™ SCP and timsTOF Ultra series
- Bruker timsTOF™ Flex Bruker Impact II
- Thermo Scientific™ Orbitrap Astral series
- Thermo Scientific™ Orbitrap Exploris™ 480
- Thermo Scientific™ Q Exactive™ Series
- Thermo Scientific™ Orbitrap Fusion™ Series
- SCIEX ZenoTOF 7600 System
- SCIEX TripleTOF® Series
- Waters Xevo® G2-XS
Supported Methods
Spectronaut supports a wide variety of DIA data acquisition methods, including but not limited to dia-PASEF, diagonal-PASEF, SWATH and FAIMS-DIA.
The minimum requirements for supported methods are a reversed-phase chromatography with either a linear or nonlinear gradient that spans at least 10-35% acetonitrile. Methods acquiring MS1 and MS2 scans are recommended, but ones acquiring only MS2 scans are also supported (in the library-based DIA analysis approach only). The cycle time of the DIA method should aim to sample chromatographic peaks sufficiently. Finally, MS1 as well as MS2 scan ranges can be segmented and data can be recorded with fractionation, including gas-phase fractionation.
For more information on supported DIA acquisition methods, please refer to the user manual.
Recommended System Requirements
Spectronaut is available for Windows and Linux operating systems (operated via command line).
Detailed, recommend specifications:
- Windows 11 x64 / Windows Server 2016 or late /Ubuntu 22.04 LTS
- Intel or AMD CPU with 16 cores or more
- 2 TB of free hard drive space or more (2x data set size)
- 16 GB of memory or more (1 precursor in 1 run amounts to ~0.5 KB of RAM)
- .NET 8.0 or higher /.NET 8.0 for Linux
*for a library-free experiment with 200 DIA runs and typical search space settings
Licensing Options
Flexible licensing is available for local, cloud, or server deployments of Spectronaut.
- Standard License: Best for small teams; includes one software instance on Windows or Linux.
- Advanced License: Includes five independent seats for local or cloud use, with support for up to five parallel analyses.
- Premium License: Customizable seat count for large-scale, parallelized analysis on cloud or local infrastructure.
Introducing Spectronaut® 20
Join Us at ASMS for the Launch of Spectronaut 20
Be among the first to experience the next generation of data analysis – register now for our exclusive Breakfast Seminar at this year’s ASMS (American Society for Mass Spectrometry). Discover new features, connect with our experts, and see how Spectronaut 20 is setting a new standard in proteomics.
What users say about Spectronaut®?
Spectronaut is an excellent addition to our informatics capabilities, especially its thorough analysis of large-scale DIA phosphopeptide data and advanced modification localization.
Spectronaut is our preferred DIA software due to its high performance and user-friendly interface, which makes analyzing complex multiplex DIA datasets easy and reliable.
We have trusted Spectronaut with our DIA workflows for over 5 years. With Spectronaut, we know we get reliable results quickly and efficiently every time. This makes Spectronaut the ideal long-term solution for our DIA projects.
These companies trust us
Frequently Asked Questions
What is Spectronaut?
Spectronaut is Biognosys’ flagship software and has been the gold standard for DIA proteomics for over a decade.
Spectronaut features two powerful approaches to DIA analysis: a classical library-based approach and directDIA, a proprietary workflow, and top-performing library-free DIA analysis.
Spectronaut performs all steps of DIA data analysis, from empirical search space generation through DIA data identification and extraction of quantitative values, followed by post-analysis with extensive data visualization and reporting.
Biognosys is committed to providing cutting-edge solutions to our users via yearly major releases of Spectronaut, launched at the ASMS conference, and interim ad-hoc updates to provide performance enhancements and address any issues quickly.
How can I request a free Spectronaut trial, and what is included during the 30-day evaluation period?
Biognosys offers a complimentary 30-day trial of Spectronaut, including full access to all features, training materials, and expert support.
During the trial, you can explore key workflows using your own DIA data or a demo dataset, with guidance available through video tutorials and a dedicated consultation session.
To request a trial, simply complete the “Start a free trial” form – our team will be in touch with next steps.
Our support team is available at support@biognosys.com.
Need help with Spectronaut?
As a Spectronaut user, there are several resources available to help you get the most out of the software:
- Additional FAQs: Explore our full FAQ.
- Video Tutorials: Explore our library of video tutorials, offering in-depth discussions and step-by-step walkthroughs of the most commonly used workflows in Spectronaut.
User Manual: For detailed guidance, refer to the software manual, available both online and within the application under the About menu.
If you need further assistance, please contact us at support@biognosys.com.
For consultations or help with specific software questions, kindly include details about your analysis, workflow steps, and any relevant error messages or logs to help us provide efficient and targeted support.
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