Spectronaut® - Biognosys

Spectronaut®

Powerful software package for comprehensive analysis of data-independent acquisition (DIA) proteomics experiments.
1st

Commercial DIA software

1000+

Users trust Spectronaut

4500+

Papers cite Spectronaut

15+

Years of software development

The gold standard for DIA proteomics analysis

Spectronaut is a high-performance DIA proteomics software solution engineered to deliver unparalleled throughput and precision. Its state-of-the-art signal processing and artificial intelligence (AI) augmentation ensure reproducible, high-accuracy quantification, while comprehensive visualization and flexible, customizable reporting facilitate in-depth data interrogation. Experience unmatched processing speed and technical rigor that empower your lab to push the boundaries of proteomics data analysis. 

Technical Details

Specifications

Supported Instruments

Spectronaut supports DIA mass spectrometry data collection collected using instruments from Bruker, Thermo Scientific, SCIEX, and Waters.

Specific supported models include: 

  • Bruker timsTOF™ Pro series and timsTOF HT
  • Bruker timsTOF™ SCP and timsTOF Ultra series
  • Bruker timsTOF™ Flex Bruker Impact II
  • Thermo Scientific™ Orbitrap Astral series
  • Thermo Scientific™ Orbitrap Exploris™ 480
  • Thermo Scientific™ Q Exactive™ Series
  • Thermo Scientific™ Orbitrap Fusion™ Series
  • SCIEX ZenoTOF 7600 System
  • SCIEX TripleTOF® Series
  • Waters Xevo® G2-XS

Spectronaut supports a wide variety of DIA data acquisition methods, including but not limited to dia-PASEF, diagonal-PASEF, SWATH and FAIMS-DIA. 

The minimum requirements for supported methods are a reversed-phase chromatography with either a linear or nonlinear gradient that spans at least 10-35% acetonitrile. Methods acquiring MS1 and MS2 scans are recommended, but ones acquiring only MS2 scans are also supported (in the library-based DIA analysis approach only). The cycle time of the DIA method should aim to sample chromatographic peaks sufficiently. Finally, MS1 as well as MS2 scan ranges can be segmented and data can be recorded with fractionation, including gas-phase fractionation.

For more information on supported DIA acquisition methods, please refer to the user manual.

Spectronaut is available for Windows and Linux operating systems (operated via command line).

Detailed, recommend specifications:

  • Windows 11 x64 / Windows Server 2016 or late /Ubuntu 22.04 LTS
  • Intel or AMD CPU with 16 cores or more 
  • 2 TB of free hard drive space or more (2x data set size) 
  • 16 GB of memory or more (1 precursor in 1 run amounts to ~0.5 KB of RAM) 
  • .NET 8.0 or higher /.NET 8.0 for Linux
    *for a library-free experiment with 200 DIA runs and typical search space settings 

Flexible licensing is available for local, cloud, or server deployments of Spectronaut.

  • Standard License: Best for small teams; includes one software instance on Windows or Linux.
  • Advanced License: Includes five independent seats for local or cloud use, with support for up to five parallel analyses.
  • Premium License: Customizable seat count for large-scale, parallelized analysis on cloud or local infrastructure. 
New update

Introducing Spectronaut® 20

Join Us at ASMS for the Launch of Spectronaut 20

Be among the first to experience the next generation of data analysis – register now for our exclusive Breakfast Seminar at this year’s ASMS (American Society for Mass Spectrometry). Discover new features, connect with our experts, and see how Spectronaut 20 is setting a new standard in proteomics.

What users say about Spectronaut®?

These companies trust us

Kuiper, our new search engine, shows the power of approaching a challenge with a fresh perspective. Tailor-made to address the complexities of unspecific search spaces in proteomics, Kuiper offers a remarkable reduction in analysis time while boosting peptide identification rates. We believe that Kuiper will contribute significantly to drive meaningful advances in peptidomics, particularly in the field of immunopeptidomics.
Lukas Reiter, PhD, CTO of Biognosys

Frequently Asked Questions

Spectronaut is Biognosys’ flagship software and has been the gold standard for DIA proteomics for over a decade.

Spectronaut features two powerful approaches to DIA analysis: a classical library-based approach and directDIA, a proprietary workflow, and top-performing library-free DIA analysis. 

Spectronaut performs all steps of DIA data analysis, from empirical search space generation through DIA data identification and extraction of quantitative values, followed by post-analysis with extensive data visualization and reporting.

Biognosys is committed to providing cutting-edge solutions to our users via yearly major releases of Spectronaut, launched at the ASMS conference, and interim ad-hoc updates to provide performance enhancements and address any issues quickly.

Biognosys offers a complimentary 30-day trial of Spectronaut, including full access to all features, training materials, and expert support.

During the trial, you can explore key workflows using your own DIA data or a demo dataset, with guidance available through video tutorials and a dedicated consultation session.

To request a trial, simply complete the “Start a free trial” form – our team will be in touch with next steps.

Our support team is available at support@biognosys.com.

As a Spectronaut user, there are several resources available to help you get the most out of the software:

  • Additional FAQs: Explore our full FAQ.
  • Video Tutorials: Explore our library of video tutorials, offering in-depth discussions and step-by-step walkthroughs of the most commonly used workflows in Spectronaut.
  • User Manual: For detailed guidance, refer to the software manual, available both online and within the application under the About menu.

If you need further assistance, please contact us at support@biognosys.com.

For consultations or help with specific software questions, kindly include details about your analysis, workflow steps, and any relevant error messages or logs to help us provide efficient and targeted support.

SpectroMineTM Manual

SpectroMineTM Tutorial

Dedicated to Spectronaut®

Resources

Eliminate Data Processing Bottlenecks with Scalable Solutions Using Spectronaut® in the Cloud
Data-driven Optimization of the Chromatographic Peak Sampling in dia-PASEF for Optimal Discovery Workflows
A Comprehensive Solution for PTM Analysis: Integrating Input Normalization and Improved Stoichiometry Calculations

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