This seminar at ASMS 2020 Reboot, had two exceptional speaker: Prof. Jesper Olsen and Prof. Florian Meier. First, Prof. Olsen shows, among other great results, how the combination of FAIMS with single CVs, data independent acquisition (DIA) and the new directDIA 2.0 in Spectronaut 14 provides, without any library, results that are comparable to the ones obtained using comprehensive project-specific libraries in older versions of Spectronaut. Then, Prof. Meier elaborates on the benefits of recently developed acquisition methods in DIA mode, supported in Spectronaut 14, such as BoxCar DIA and dia-PASEF. These are specifically thought for optimizing ion acquisition to help overcome traditional limitations such as high dynamic range and scarce samples.
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