Services

World leading contract research services for high content protein quantification and analysis

Biognosys offers the most advanced proteomics services for high content protein analysis

We provide contract research services for high-content protein quantification from any species in any biological matrix to facilitate translational research. Whatever your protein analysis need is, be the analysis of entire proteomes or quantification of specific proteins, Biognosys has the right solution for you. With our market leading expertise in next-generation proteomics we are a strong partner to accelerate your research and development.

The Biognosys team is ready to support you and we are happy to develop a research plan with you, tailored to your research objectives. Our projects are carried out in our state of the art proteomics facility. Should you wish to implement our methods in your in-house proteomics facility we assist you with method transfer and provide the needed reagents and bioinformatics tools.

Depending on your research question Biognosys offers two main label free protein quantification platforms:

  1. DISCOVERY proteomics enables profiling of up to 9’000 proteins per sample across treatments or conditions and identifies significantly regulated proteins. This platform is ideal for studies where the main question is “What happens to my proteome?”
  2. TARGETED proteomics allows high-throughput absolute quantification of up to 150 proteins in hundreds or thousands of samples. It is ideal for researchers asking “What happens to my proteins of interest?”

DISCOVERY and TARGETED proteomics bridge the gap between scale and accuracy in protein analysis that opens up between antibody-based methods and classical “shotgun” proteomics. Whereas antibody-based methods (such as ELISA) allow accurate quantification of single proteins they are difficult to “multiplex”, i.e. to measure multiple proteins in complex samples in a single experiment. Conversely, classical “shotgun” proteomics identifies many proteins in one experiment, but the methods lack of reproducibility makes it hard to reliably quantify many proteins across samples.

 

Discovery Proteomics

Discovery proteomics solutions rely on Hyper Reaction Monitoring (HRM-MS™), a Next Generation proteomics technology invented at Biognosys for quantification of complete proteomes with mass spectrometry. HRM enables reproducible and precise quantification of thousands of proteins in a single instrument run with data independent acquisition (DIA or SWATH). Several standardized proteomics services are available to help meet your particular discovery proteomics needs:

 

ID

  • Proven HRM workflow
  • Deep spectral library
  • Time and cost efficient

Ideal for typical sized projects with expectations of high proteome coverage delivered in a timely and cost efficient manner.

ID+

  • Elongated gradient for a deeper analysis with more identified proteins
  • Deep spectral library

Ideal for a comprehensive proteomic analysis of your samples.

HighD

  • Maximum depth of analysis and highest number of identified proteins with longest gradient
  • Deep spectral library

Ideal for the most extensive proteome profiling studies.

SpeeD

  • High throughput option for large projects
  • Deep spectral library

Ideal for rapidly screening through a large number of samples (>96) in a timely and cost efficient manner. Especially suited for bio fluid samples such as plasma, urine or similar. 

 

Post-translational modification analysis or other customized service are also available upon request. Discovery proteomics analysis is available for virtually any species and any biological matrix. Detailed information for the most frequently requested sample types:

 

  Cell culture Blood Tissue Urine CSF
Sample preparation protocols available yes yes yes yes yes
# proteins typically identified in sample 4000-8000 300-800 4000-8000 600-1200 400-900
Sample amount required 105-106 cells 5 ul 5-10 mg 100-200 ul 50-100 ul
PTM analysis available Phoshpo
Glyco
Acetylation
Custom
Glyco
Custom
Phoshpo
Acetylation
Custom
- Glyco
Custom

 

Examples of discovery projects carried out:

  • Discovery of differentially regulated proteins in human CSF samples from two patient groups
  • Secreted protein identification in primary cell supernatants upon antibody drug treatment
  • Discovery of activated pathways in mouse liver samples upon drug treatment
  • Large scale biomarker discovery from urine in > 120 samples

 

 Read about the revolutionary Next Generation proteomics - Hyper Reaction Monitoring (HRM) in our technology section.

 Learn about Biognosys tools that enable you to transfer the HRM technology to your own lab.

 

 

Targeted Proteomics

Multiple and Parallel Reaction Monitoring (MRM and PRM) are targeted proteomics techniques that offer highly specific and sensitive multiplexed quantification of selected proteins from complex biological samples. Biognosys offers a targeted proteomics services for absolute or relative quantification of up to 150 target proteins per run with a dynamic range of 6 orders of magnitude:

 

Relative Quant

  • Customized assay panel development and sample measurement.
  • Possibility to transfer to your facility.

Absolute Quant

  • Customized assay panel development and sample measurement including stable isotope peptides for absolute quantification.
  • Possibility to transfer to your facility.

PlasmaDive

  • Plasma sample measurement using a pre-configured assay panel for 100 human plasma proteins. Also available as a ready-to-use kit for your in-house facilty.

PlasmaDeepDive

  • Plasma sample measurement using a pre-configured assay panel for 100 low-abundant human plasma proteins. Also available as a ready-to-use kit for your in-house facilty.

 

Targeted proteomics analysis is available for virtually any species and any biological matrix. Detailed information for the most frequently requested sample types:

 

  Cell culture Blood Tissue Urine CSF
Sample preparation protocols available yes yes yes yes yes
Sample amount required 105-106 cells 5 ul 5-10 mg 100-200 ul 50-100 ul

 

Examples of targeted proteomics projects carried out:

  • High-throughput MRM method development for protein quantification in human cell culture samples
  • Development of an MRM panel for quantification of cardiovascular disease markers
  • Development of a multiplexed MRM panel for pathway modeling in Maize

 

 Read about the powerful targeted proteomics technology – Multiple and Parallel reaction monitoring in our technology section.

 Learn about Biognosys tools that enable you to transfer the targeted proteomics technology to your own lab.

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