Singh J, Kaade E, Muntel J, Bruderer R, Reiter L, Thelen M, Winter D. Journal of Proteome Research.
The lysosome is a critical cell compartment dedicated to the recycling of macromolecules and organelles, and it is associated with rare inherited fatal disorders. The analysis of lysosomal proteins by DIA requires a protocol for the enrichment of the lysosome fraction in a sample. In collaboration with the Winter lab at the University of Bonn, we achieved a high number of protein identifications and quantitative reproducibility by means of nanoparticle- and immunoprecipitation-based enrichment strategies.
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