Systematic Comparison of Strategies for the Enrichment of Lysosomes by Data Independent Acquisition - Biognosys

Systematic Comparison of Strategies for the Enrichment of Lysosomes by Data Independent Acquisition

Publications

Singh J, Kaade E, Muntel J, Bruderer R, Reiter L, Thelen M, Winter D. Journal of Proteome Research.

The lysosome is a critical cell compartment dedicated to the recycling of macromolecules and organelles, and it is associated with rare inherited fatal disorders. The analysis of lysosomal proteins by DIA requires a protocol for the enrichment of the lysosome fraction in a sample. In collaboration with the Winter lab at the University of Bonn, we achieved a high number of protein identifications and quantitative reproducibility by means of nanoparticle- and immunoprecipitation-based enrichment strategies.

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