Baeza J, Lawton AJ, Fan J, Smallegan MJ, Lienert I, Gandhi T, Bernhardt OM, Reiter L, Denu JM. Journal of Proteome Research.
In this study, the Biognosys’ R&D team, in collaboration with researchers of the University of Wisconsin-Madison, co-developed and benchmarked a DIA method to quantify acetylation stoichiometry in the cell proteome. This improved DIA method overcomes the limitations of DDA, allowing the accurate and reproducible quantification of light and heavy acetyl-lysine fragment ions. The method was benchmarked on synchronized cell lines with defined acetylation stoichiometry, providing a detailed landscape of the site-specific acetylation dynamics across cellular compartments.