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Discovery proteomics software for the targeted analysis of DIA measurements from various MS platforms.

Spectronaut is the most advanced and versatile software for DIA proteomics, providing more identifications with a higher level of confidence.

Please take a look at our General Software License.


Product number: Sw-3001

Spectronaut™ is a commercial software package aimed at analyzing data-independent acquisition (DIA) proteomics experiments. Spectronaut can quantitatively profile 100s to several 1000s of proteins in one experiment. Large experiments with several conditions and replicates consisting of up to 10000s of LC-MS runs can be analyzed.

Some of Spectronaut's features are:

  1. Vendor Independent: Spectronaut supports DIA data from any platform
  2. Usability: Effortless and robust DIA data analysis with setup wizards and automatic detection of optimal parameters
  3. Complete Workflow: Spectronaut provides a holistic solution for DIA experiment, from library generation to biological interpretation
  4. Performance and Scalability: The fastest solution that allows you to analyze 10,000s of runs with large libraries
  5. Versatility and Flexibility: Spectronaut quantifies label-free, labeled and spike-in experiments


To learn more about the possibilities Spectronaut offers, contact us at 

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Supported Instruments

  • Thermo Scientific™ Orbitrap Exploris™ 480
  • Thermo Scientific™ Q Exactive™ Series
  • Thermo Scientific™ Orbitrap Fusion™ Series
  • SCIEX TripleTOF® Series
  • Bruker Impact II
  • Bruker timsTOF™ and timsTOF™ Pro*
  • Waters Xevo® G2-XS

Supported Methods

Spectronaut™ analyzes DIA data from a large variety of different methods:

  • HRM™
  • BoxCar DIA
  • SWATH™
  • SWATH™ 2.0
  • SONAR™
  • dia-PASEF®

Methods should acquire MS1 and MS2 or MS2 only scans. The cycle time of the method should be in the range of 1-3 seconds depending on your average peak width. Fractionation, including gas phase fractionation, is supported.


Minimum System Requirements

  • Windows 7 x64
  • Intel Core™ CPU 2.7 GHz (quad core) or similar
  • 500 GB of free HDD space
  • 16 GB of RAM memory
  • .NET Framework 4.7


Expected memory consumption


How to Order

Please submit a request form. Our team will review your inquiry and get back to you within 5 working days.



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Spectronaut 14 Launch Page

Spectronaut 14 Launch Seminar

Spectronaut™ Brochure

Spectronaut™ Video Tutorials

Spectronaut™ Manual

FASTA of iRT peptides for shotgun search

Skyline Schema for exporting Spectronaut compatible libraries - simply add this schema to Skyline's report list


Demo Data Files

Spectronaut Demo Data Set (zip file, 1.6 GB)


  • Six run files
  • FASTA file
  • Spectral library file
  • Condition setup file
  • Gene Ontology Annotation file 

Spectronaut Experiment File (sne file, 1.05 GB)


Code Project

BGMS Raw API - Example Code Project

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Let us help you do great proteomics!

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