Simple-to-use standardized sample preparation kit for reproducible mass spectrometry proteomics
Variations in sample preparation between experiments and individual samples are an enormous source of experimental bias in proteomics, especially in high-throughput applications. Since the proteomes of natural samples are extremely complex with typically 100’000s of peptides present, spanning over several orders of magnitude, it is challenging to establish a reliable method for preparing protein samples for analysis by mass spectrometry.
Biognosys’ Sample Preparation Kit is a simple-to-use standardized kit for reproducible mass spectrometry proteomics of mammalian cell cultures and plasma/serum samples. The protocols work well with all mass spectrometry based proteomics applications such as SRM/MRM, HRM/DIA/SWATH and shotgun MS/MS.
Sample Preparation Kit Pro components
Alkylation Solution (1x 10 ml tube)
Reduction Stock Solution (1x 0.5 ml tube)
LC Solution (2x 10 ml tube)
10x Dilution Buffer (1x 10 ml tube)
Denature Buffer (1x 10 ml tube)
The kit components are sufficient for 48 plasma or 48 mammalian cell culture sample preparations to be made at once. It requires 10 µl per sample of plasma/serum or mammalian cell culture pellets with at least 100 µg of total protein per sample.
The Sample Preparation Kit manual is designed to support users. It provides Kit components and technicalities and recommended protocols for reproducible sample preparation compatib [...]
Sample Preparation Kit Quick Reference Card