Sample Preparation Kit - Biognosys
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Sample Preparation Kit

300.00

Simple-to-use standardized sample preparation kit for reproducible mass spectrometry proteomics

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300.00 €
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Variations in sample preparation between experiments and individual samples are an enormous source of experimental bias in proteomics, especially in high-throughput applications. Since the proteomes of natural samples are extremely complex with typically 100’000s of peptides present, spanning over several orders of magnitude, it is challenging to establish a reliable method for preparing protein samples for analysis by mass spectrometry.

Biognosys’ Sample Preparation Kit is a simple-to-use standardized kit for reproducible mass spectrometry proteomics of mammalian cell cultures and plasma/serum samples. The protocols work well with all mass spectrometry based proteomics applications such as SRM/MRM, HRM/DIA/SWATH and shotgun MS/MS.

Understanding the target of a small molecule compound is key to gaining deeper insight into its mechanism of action and potential toxicities.

Limited Proteolysis (LiP-MS) has emerged as a label-free technology to identify and analyze changes in protein structures/steric hindrance caused by a compound in cellular lysates. The underlying concept of LiP-MS is based on proteins being subjected to proteolysis in the presence or absence of a compound. When a compound binds to a protein, it induces a specific structural state in the protein target that can be exploited during proteolysis. This unique structural state changes the pattern of proteolysis and leads to the formation of unique peptides that can be characterized by HRM mass spectrometry.

Understanding the target of a small molecule compound is key to gaining deeper insight into its mechanism of action and potential toxicities.

Understanding the target of a small molecule compound is key to gaining deeper insight into its mechanism of action and potential toxicities.

Understanding the target of a small molecule compound is key to gaining deeper insight into its mechanism of action and potential toxicities.

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