Peptide Kits
PlasmaDeepDive™ Reference Peptides
Reference peptides for absolute quantification of 100 proteins in depleted human plasma / serum samples
Includes transition list for method set-up and data analysis
Available for 48 or 96 samples
PlasmaDeepDive™ Reference Peptides 48
Product number: Ki-3017-48
USD
2,750.00
In stock
PlasmaDeepDive™ Reference Peptides 96
Product number: Ki-3017-96
USD
5,000.00
In stock
PlasmaDeepDive™ Reference enables absolute quantification of 100 human plasma proteins in a single measurement, covering a dynamic range of 6 orders of magnitude with high precision and reproducibility. It is specifically designed to monitor protein levels in depleted plasma or serum and sets a new standard for clinical research, biomarker screening and development of companion diagnostics.
PlasmaDeepDive™ Mini Multiplexed Assay Panel combines 100 peptide assays in one multiplexed, scheduled MRM or PRM method. It includes 16 peptides corresponding to the typically depleted proteins to monitor the depletion quality, 9 housekeeping proteins for normalization and consequently high reproducibility of the results, and 75 proteins that represent relevant targets for biomarker screening.
PlasmaDeepDive™ Reference Peptides Kit contains
- Stable isotope standards for accurate quantitation
- iRT Standard for transfer to virtually any LC setup
- Transition list for the stable isotope standards
List of Proteins
Entry Name |
UniPot ID |
Description |
|
ADIPO_HUMAN |
Q15848 |
Adiponectin |
|
ALS_HUMAN |
P35858 |
Insulin-like growth factor-binding complex acid labile subunit |
|
BDNF_HUMAN |
P23560 |
Brain-derived neurotrophic factor |
|
C1RL_HUMAN |
Q9NZP8 |
Complement C1r subcomponent-like protein |
|
CAD13_HUMAN |
P55290 |
Cadherin-13 |
|
CADH5_HUMAN |
P33151 |
Cadherin-5 (CD144) |
|
CBPB2_HUMAN |
Q96IY4 |
Carboxypeptidase B2 |
|
CD14_HUMAN |
P08571 |
Monocyte differentiation antigen CD14 |
|
CH3L1_HUMAN |
P36222 |
Chitinase-3-like protein 1 |
|
CHL1_HUMAN |
O00533 |
Neural cell adhesion molecule L1-like protein |
|
CNDP1_HUMAN |
Q96KN2 |
Beta-Ala-His dipeptidase |
|
CNTF_HUMAN |
P26441 |
Ciliary neurotrophic factor |
|
CO4B_HUMAN |
P0C0L5 |
Complement C4-B |
|
CRAC1_HUMAN |
Q9NQ79 |
Cartilage acidic protein 1 |
|
CRF_HUMAN |
P06850 |
Corticoliberin |
|
CXCL7_HUMAN |
P02775 |
Platelet basic protein |
|
CYTC_HUMAN |
P01034 |
Cystatin-C |
|
DIAC_HUMAN |
Q01459 |
Di-N-acetylchitobiase |
|
DOPO_HUMAN |
P09172 |
Dopamine beta-hydroxylase |
|
EGFR_HUMAN |
P00533 |
Epidermal growth factor receptor |
|
ENOG_HUMAN |
P09104 |
Gamma-enolase |
|
FA11_HUMAN |
P03951 |
Coagulation factor XI |
|
FBLN3_HUMAN |
Q12805 |
EGF-containing fibulin-like extracellular matrix protein 1 |
|
GFAP_HUMAN |
P14136 |
Glial fibrillary acidic protein |
|
GP1BA_HUMAN |
P07359 |
Platelet glycoprotein Ib alpha chain |
|
GPV_HUMAN |
P40197 |
Platelet glycoprotein V (CD42d) |
|
GRP75_HUMAN |
P38646 |
Stress-70 protein, mitochondrial |
|
GRP78_HUMAN |
P11021 |
78 kDa glucose-regulated protein |
|
HGFA_HUMAN |
Q04756 |
Hepatocyte growth factor activator |
|
HS12A_HUMAN |
O43301 |
Heat shock 70 kDa protein 12A |
|
HS71L_HUMAN |
P34931 |
Heat shock 70 kDa protein 1-like |
|
HS74L_HUMAN |
O95757 |
Heat shock 70 kDa protein 4L |
|
HSP71_HUMAN |
P08107 |
Heat shock 70 kDa protein 1A/1B |
|
HSP74_HUMAN |
P34932 |
Heat shock 70 kDa protein 4 |
|
HSP76_HUMAN |
P17066 |
Heat shock 70 kDa protein 6 |
|
HSP7C_HUMAN |
P11142 |
Heat shock cognate 71 kDa protein |
|
HSP7E_HUMAN |
Q0VDF9 |
Heat shock 70 kDa protein 14 |
|
IBP2_HUMAN |
P18065 |
Insulin-like growth factor-binding protein 2 |
|
IBP3_HUMAN |
P17936 |
Insulin-like growth factor-binding protein 3 |
|
IDHC_HUMAN |
O75874 |
Isocitrate dehydrogenase [NADP] cytoplasmic |
|
IGF1_HUMAN |
P05019 |
Insulin-like growth factor I |
|
IGF2_HUMAN |
P01344 |
Insulin-like growth factor II |
|
IL10_HUMAN |
P22301 |
Interleukin-10 |
|
IL1AP_HUMAN |
Q9NPH3 |
Interleukin-1 receptor accessory protein |
|
ITIH3_HUMAN |
Q06033 |
Inter-alpha-trypsin inhibitor heavy chain H3 |
|
LBP_HUMAN |
P18428 |
Lipopolysaccharide-binding protein |
|
LDHB_HUMAN |
P07195 |
L-lactate dehydrogenase B chain |
|
LEP_HUMAN |
P41159 |
Leptin |
|
LUM_HUMAN |
P51884 |
Lumican |
|
LYAM1_HUMAN |
P14151 |
L-selectin (CD62L) |
|
MGMT_HUMAN |
P16455 |
Methylated-DNA--protein-cysteine methyltransferase |
|
MINP1_HUMAN |
Q9UNW1 |
Multiple inositol polyphosphate phosphatase 1 |
|
MMP9_HUMAN |
P14780 |
Matrix metalloproteinase-9 |
|
MMRN1_HUMAN |
Q13201 |
Multimerin-1 |
|
NCAM1_HUMAN |
P13591 |
Neural cell adhesion molecule 1 (CD56) |
|
NEBU_HUMAN |
P20929 |
Nebulin |
|
NEST_HUMAN |
P48681 |
Nestin |
|
NEUT_HUMAN |
P30990 |
Neurotensin/neuromedin N |
|
NGF_HUMAN |
P01138 |
Beta-nerve growth factor |
|
NMB_HUMAN |
P08949 |
Neuromedin-B |
|
NPY_HUMAN |
P01303 |
Pro-neuropeptide Y |
|
NRP1_HUMAN |
O14786 |
Neuropilin-1 (CD304) |
|
P53_HUMAN |
P04637 |
Cellular tumor antigen p53 |
|
PHLD_HUMAN |
P80108 |
Phosphatidylinositol-glycan-specific phospholipase D |
|
PROZ_HUMAN |
P22891 |
Vitamin K-dependent protein Z |
|
PTEN_HUMAN |
P60484 |
Phosphatase and tensin homolog (PTEN) |
|
PZP_HUMAN |
P20742 |
Pregnancy zone protein |
|
RETN_HUMAN |
Q9HD89 |
Resistin |
|
SAMP_HUMAN |
P02743 |
Serum amyloid P-component |
|
SYPL1_HUMAN |
Q16563 |
Synaptophysin-like protein 1 |
|
TIMP1_HUMAN |
P01033 |
Metalloproteinase inhibitor 1 |
|
TKN1_HUMAN |
P20366 |
Protachykinin-1 |
|
TSP1_HUMAN |
P07996 |
Thrombospondin-1 |
|
VASN_HUMAN |
Q6EMK4 |
Vasorin |
|
VWF_HUMAN |
P04275 |
von Willebrand factor |
|
A1AT_HUMAN |
P01009 |
Alpha-1-antitrypsin |
|
A2MG_HUMAN |
P01023 |
Alpha-2-macroglobulin |
|
ALBU_HUMAN |
P02768 |
Serum albumin |
|
APOA1_HUMAN |
P02647 |
Apolipoprotein A-I |
|
APOA2_HUMAN |
P02652 |
Apolipoprotein A-II |
|
CO3_HUMAN |
P01024 |
Complement C3 |
|
FIBA_HUMAN |
P02671 |
Fibrinogen alpha chain |
|
FIBB_HUMAN |
P02675 |
Fibrinogen beta chain |
|
FIBG_HUMAN |
P02679 |
Fibrinogen gamma chain |
|
HPT_HUMAN |
P00738 |
Haptoglobin |
|
IGHA1_HUMAN |
P01876 |
Ig alpha-1 chain C region |
|
IGHG1_HUMAN |
P01857 |
Ig gamma-1 chain C region |
|
IGHG2_HUMAN |
P01859 |
Ig gamma-2 chain C region |
|
IGHM_HUMAN |
P01871 |
Ig mu chain C region |
|
TRFE_HUMAN |
P02787 |
Serotransferrin (Transferrin) |
|
TTHY_HUMAN |
P02766 |
Transthyretin |
|
A1BG_HUMAN |
P04217 |
Alpha-1B-glycoprotein |
|
CERU_HUMAN |
P00450 |
Ceruloplasmin |
|
HEMO_HUMAN |
P02790 |
Hemopexin |
|
IC1_HUMAN |
P05155 |
Plasma protease C1 inhibitor |
|
ITIH2_HUMAN |
P19823 |
Inter-alpha-trypsin inhibitor heavy chain H2 |
|
ITIH4_HUMAN |
Q14624 |
Inter-alpha-trypsin inhibitor heavy chain H4 |
|
KNG1_HUMAN |
P01042 |
Kininogen-1 |
|
VTDB_HUMAN |
P02774 |
Vitamin D-binding protein |
|
VTNC_HUMAN |
P04004 |
Vitronectin |
Please contact us for support.
PlasmaDeepDive™ Reference Peptides contains stable isotope standards with integrated iRT peptides for accurate quantitation and Panel Plug-in for method set-up and data analysis.
PlasmaDeepDive™ Reference Peptides 48 contains:
- Reference Peptide Mix (1x 1.1 ml glass vial)
- Dissolution Buffer (1x 2.0 ml tube)
- LC solution (1x 2.0 ml tube)
- PlasmaDeepDive™ transition list
PlasmaDeepDive™ Reference Peptides 96 contains:
- Reference Peptide Mix (2x 1.1 ml glass vial)
- Dissolution Buffer (1x 2.0 ml tube)
- LC solution (1x 2.0 ml tube)
- PlasmaDeepDive™ transition list
Please contact us for support.
Biognosys Tools for Blood Proteomics Brochure
PlasmaDeepDive™ Reference Peptides Manual
PlasmaDeepDive™ Reference Peptides 96 Kit Quick Reference Card
PlasmaDeepDive™ Reference Peptides 48 Kit Quick Reference Card
Please contact us for support.
Visit Science Hub to access all scientific and technical content (publications, videos, application notes, posters and more) related to Biognosys technology, products and services.
Peer-reviewed papers
- Yin X, Baig F, Haudebourg E, Blankley RT, Gandhi T, et al. Plasma Proteomics for Epidemiology: Increasing Throughput With Standard-Flow Rates. Circ Cardiovasc Genet. 2017 Dec;10(6).
- Kiseleva OI, Romashova YA, Moskaleva NE, Petushkova NA, Teryaeva NB, Belyaev AY, Lisitsa AV. Plasma preparation to measure FDA-approved protein markers by selected reaction monitoring. Clin Transl Med. 2015; 4(1):32.
Please contact us for support.
What is the difference between PlasmaDive™ and PlasmaDeepDive™ Multiplexed Assay Panels and PlasmaDive™ and PlasmaDeepDive™ Reference Peptides?
PlasmaDive™, PlasmaDive™ Mini, PlasmaDeepDive™ and PlasmaDeepDive™ Mini are comprehensive Multiplexed Assay Panels that contain reagents, plastic ware, optimized protocol for reproducible sample preparation, stable isotope standards with integrated iRT peptides for accurate quantitation, Panel Plug-in for method set-up and data analysis software SpectroDive™.
On the other hand, PlasmaDive™ and PlasmaDeepDive™ Reference Peptides contain only the stable isotope reference peptides for either 48 or 96 samples and Panel Plug-in for method set-up and data analysis. These solutions can be easily integrated in your own sample preparation protocol and data analysis software.
What is the difference between PlasmaDive™, PlasmaDive™ Mini and PlasmaDeepDive™ and PlasmaDeepDive™ Mini Multiplexed Assay Panels?
PlasmaDive™ and PlasmaDive™ Mini monitor an identical set of 100 proteins levels in human plasma or serum samples with high throughput. Each stable isotope standard peptide represents a human plasma protein, with almost half of the panel’s proteins being considered a putative cancer, cardio-vascular or metabolic biomarker. The difference is only in the number of samples that can be processed. PlasmaDive™ is made for 48 or 96 samples and PlasmaDive™ Mini is made for 20 samples.
PlasmaDeepDive™ and PlasmaDeepDive™ Mini multiplexed assay panel target 100 proteins to be measured in one scheduled MRM or PRM experiment. It includes 16 peptides corresponding to the typically depleted proteins in human plasma analysis, which can be used to monitor the depletion quality, 9 housekeeping proteins for normalization and consequently high reproducibility of the results, and 75 proteins that represent relevant targets for biomarker screening. The difference is only in the number of samples that can be processed. PlasmaDeepDive is made for 48 or 96 samples and PlasmaDeepDiveMini is made for 20 samples.
What is the dynamic range of PlasmaDive™ and PlasmaDeepDive™ combined?
Together the two kits span 6-7 orders of magnitude dynamic range with high precision and reproducibility. Additional information on the targeted proteins and use cases can be found in Science Hub section of the Biognosys website.
Can I measure only a subset of the proteins in each kit?
Of course, assays of only a subset of the proteins in one kit can be generated and measured. Thereby the measurement time can be reduced and the sensitivity of the analysis increased.
Can I use the PlasmaDeepDive™ reference peptides with native (not depleted) plasma?
Yes, this is possible. However, sample preparation protocol needs to be updated and you should be aware that some of the analytes in PlasmaDeepDive™ are low abundant proteins though and might not be detectable in non-depleted plasma in all conditions. Please contact us for additional information.
