A single pack for 500 injections.
iRT – indexed Retention Time – is a dimensionless value that defines chromatographic retention of a peptide for a defined resin type (e.g. C18) relative to the iRT Standard. In contrast to empirical retention time, the iRT value of a peptide is stable and enables accurate prediction of peptide retention for any chromatographic setup.
The iRT Standard contains eleven non-naturally occurring synthetic peptides in a pooled mix. Peptides have been carefully optimized for stability, sensitivity and even retention time spacing over the gradient. The iRT Kit allows straightforward determination of peptide iRT values and calibration of chromatographic systems.
By calibrating your LC system with the iRT Kit you will dramatically increase the throughput of your targeted proteomics (MRM and PRM) experiments - even up to 20 times - by bundling more transitions into one run. The benefits of iRT standards are also important in the next-generation proteomic workflows such as DIA or SWATH. iRT peptides allow high-quality spectral library generation and precise normalization of your DIA or SWATH runs.
The iRT peptides work with all Biognosys software solutions: Spectronaut for DIA data analysis, SpectroDive for targeted proteomics experiments, SpectroMine for isobaric labeled quantitative experiments, and QuiC for MS performance monitoring.
The kit components are sufficient for approximately 500 injections depending on sample volume and injection amount.
The kit components are sufficient for approximately 1000 injections depending on sample volume and injection amount.
The iRT Kit is used in time resolved mass spectrometry as a two-step process:
See the Quick Reference Card for detailed information on how to use the iRT Kit.
iRT Kit reference sheet for transitions and sequence information (excel file)
FASTA of iRT peptides for shotgun search
iRT Peptides Volume Calculator (excel file)
iRT Real Sequences lists real iRT sequences and compares them to previously used nominal sequences (excel file)
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