Targeted Data Extraction of the MS/MS Spectra Generated by Data-Independent Acquisition: a New Concept for Consistent and Accurate Proteome Analysis

Gillet L, Navarro P, Tate S, Röst H, Selevsek N, Reiter L, Bonner R, Aebersold R. MCP.

This work introduced the targeted extraction of MS/MS spectra generated by DIA/SWATH using fragment ion spectral libraries. The consistency and accuracy of quantification were comparable to MRM, the gold standard in proteomics quantification. This technology marked a breakthrough in terms of single shot label free quantification in proteomics becaused it drastically reduced the number of missing values while increasing depth and precision of quantification.

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