SpectroDive™ 11: Straightforward Validation of Your Protein Targets

SpectroDive™ 11: Straightforward Validation of Your Protein Targets

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SpectroDive™ 11 makes your targeted proteomics analysis easier than ever with new features, including absolute quantification using calibration curves, ion mobility support for FAIMS-PRM and prm-PASEF, and improved peak-picking. Discover the most seamless way to validate and quantify your protein targets.

“SpectroDive boosts our productivity: it is intuitive to use, provides powerful automatic peak detection, and enables effective analysis of large datasets.”

 

Alessandro Ori, PhD, Group Leader at Leibniz Institute on Aging

Feature Overview:

 

 

 

  • New HybridDIA workflow: The PRM part of HybridDIA can now be analyzed in SpectroDive

 

  • Fully compatible with PQ500™ Reference Peptides: Simplify your plasma research by using SpectroDive for your PQ500 analysis

 

Absolute Quantification with Calibration Curves

Using calibration curves in targeted proteomics analysis allows you to accurately calculate sensitivity and quantification ranges based on precision.

 

The addition of calibration curves in SpectroDive 11 makes absolute quantification even more accurate than before. This new feature allows you to calculate calibration curves and linear ranges, as well as limits of detection and quantification.

 

SpectroDive’s calibration curves come ready to use for both analytical and clinical assays.

Improved Sensitivity with Ion Mobility

SpectroDive now offers ion mobility support for FAIMS-PRM and prm-PASEF to give you even more confidence in your targeted proteomics data.

 

Ion mobility support in SpectroDive 11 improves sensitivity and filters out unwanted interference in peptide quantification. It also offers better confidence in the identification of peptides and an added dimension to enhance peptide selection.

Tutorial: How to Perform Absolute Quantification Using Calibration Curves

Calibration curves are a valuable method to determine the concentration of an analyte in an unknown sample. To achieve this, the unknown sample is compared to a set of standard samples with known concentrations.

 

Calibration curves can also be used to determine the sensitivity of a method. This is reported by a limit of detection (LOD).

 

In this tutorial, Fabia Simona from our product support team demonstrates how to use calibration curves in SpectroDive 11 to achieve absolute quantification.

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