Quantitative profiling of HLA class I/II (neo)antigens in tissue biopsy and ​PBMC samples using an optimized mass spectrometry-based workflow

This poster emphasizes the importance of MHC molecules in immune responses, particularly in cancer research, where comprehending tumor-associated antigens holds great significance. Accurately quantifying HLA class I/II neoantigens is essential for understanding how the immune system detects and reacts to cancerous cells. However, a significant limitation lies in the high input material requirement.

We devised an integrated immunopeptidomics (IMPX) workflow tailored for cell and tissue samples to address this challenge. This innovative approach allows for in-depth profiling of immunopeptides while substantially reducing the input material needed, which is particularly beneficial for PBMC samples.