Single-Slide FFPE Proteomic Profiling Enables Therapeutic Target Quantification and Molecular Subtyping in Non-Hodgkin’s Lymphoma and Bone Marrow Tumor Biopsies

Molecular profiling of formalin-fixed paraffin-embedded (FFPE) tumors in clinical research is performed with low-plex immunohistochemistry or genome-wide transcriptomics. The application of unbiased, highly multiplexed proteomics to FFPE is limited by sample input requirements, assay throughput and complex bioinformatics. Here we report a proteomic method amenable to low-input FFPE profiling which complements transcriptomics.