Sample Preparation Advancements for Clinical Tissue Specimen for Immunopeptidomics

Modulating the immune system in the fight against cancer has become a fast growing field with notable success. The study of presented peptides on major histocompatibility complex (MHC), i.e., immunopeptides, in disease and healthy tissues have become cornerstones of immuno-oncology. Mass spectrometry is the method of choice for the study of immunopeptides. With the progression from cell lines and model systems’ basic research to clinical application, the need for robust workflows for analysis of biopsies. The minute nature of needle biopsies require small scale and single preparation sample preparation and MS acquisition. We present progress in sample preparation downscaling and its application to clinically relevant sample types.