Isoform‐resolved Correlation Analysis between mRNA Abundance Regulation and Protein Level Degradation

Salovska B, Zhu H, Gandhi T, Frank M, Li W, Rosenberger G, Wu C, Germain PL, Zhou H, Hodny Z, Reiter L, Liu Y. Molecular Systems Biology.

In a collaboration between Prof. Liu’s group at Yale University and Biognosys, we established a combined DIA-MS and pulse SILAC approach to unravel the correlation between protein degradation and mRNA abundances in HeLa cells. Among other improvements, the direct pSILAC-DIA approach offers better quantitative accuracy and richer quantitative features if compared to alternative strategies, resulting in a correlation between mRNA level and protein degradation rates to be resolved at the isoform levels.

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