Digital Proteome: a Multi-Species, Multi-Tissue Proteome Expression Atlas Supporting Target Discovery and Validation

Proteins are central mediators of drug action, functioning as both therapeutic targets and potential off-targets for small molecules and biologics. A detailed understanding of their expression profiles across tissues, in both healthy and diseased states, is essential throughout drug discovery and development.

These insights inform target accessibility, off-target liabilities, and enable early de-risking of therapeutic candidates. Traditional ligand-based methods, such as antibody-dependent assays, are limited by species specificity and lengthy development timelines. In contrast, mass spectrometry (MS)–based approaches offer a versatile, sensitive, and matrix-independent means to quantify protein expression across tissues and species on a proteome-wide scale. Building on recent advances in MS technology, we established the Digital Proteome, a comprehensive resource for protein detectability and expression profiling.