Today we would like to highlight two interesting publications that feature Biognosys’ Spectronaut™ Pulsar software for data independent acquisition (DIA) workflows.
The group of Prof. Jesper Olsen from The Novo Nordisk Foundation Center for Protein Research at University of Copenhagen, benchmarked the performance of the latest Orbitrap instrument - the Thermo Scientific™ Q Exactive™ HF-X, against its predecessor for proteomics applications. On a 30 min gradient they were able to reproducibly identify close to 6’000 proteins from a HeLa cell culture using a DIA workflow and subsequent data analysis with the Spectronaut software. The results are published in the Journal of Proteome Research under the title “Performance evaluation of the Q Exactive HF-X for shotgun proteomics”.
Researchers from Vrije Universiteit in Amsterdam performed a comparative study of three different DIA workflows, namely, classical DIA, WiSIM-DIA and untargeted DIA. While data analyses of DIA and WiSIM-DIA generally require a classical data dependent acquisition (DDA) based spectral library, recent development of untargeted DIA allows direct interrogation of raw data from a regular DIA experiment. In this case, Spectronaut Pulsar software was used to generate a spectral library from the DIA data for the untargeted DIA experiment. The study “Comparative analyses of data independent acquisition mass spectrometric approaches: DIA, WiSIM-DIA and untargeted DIA” is published in Proteomics journal.