Target Deconvolution is a novel approach based on limited proteolysis coupled to quantitative proteomics applicable for the identification of the binding protein targets of small molecules in complex proteome setting. The approach is exclusively licensed by Biognosys.
Understanding the target of a small molecule compound is key to gaining deeper insight into its mechanism of action and potential toxicities.
Limited Proteolysis (LiP-MS) has emerged as a label-free technology to identify and analyze changes in protein structures/steric hindrance caused by a compound in cellular lysates. The underlying concept of LiP-MS is based on proteins being subjected to proteolysis in the presence or absence of a compound. When a compound binds to a protein, it induces a specific structural state in the protein target that can be exploited during proteolysis. This unique structural state changes the pattern of proteolysis and leads to the formation of unique peptides that can be characterized by HRM mass spectrometry.
The Target Deconvolution service offers a fast turnaround time of 5-6 weeks per study.
Disclaimer: The Limited Proteolysis technology is patent protected and Biognosys holds an exclusive license from ETH Zürich.
Learn more about the novel Limited Proteolysis approach from the publications in the Support Materials section.
Biognosys is committed to providing the best possible results to our customers. We consider confidentiality, proactive communication, and structured project management as key factors in a successful customer relationship. Service projects are usually structured in the following way:
1. Project Set-up
2. Project Execution
3. Project Reporting
The results are presented and discussed with you in a seminar or webinar and delivered electronically in PDF and Excel formats that contain:
Learn more about the novel Limited Proteolysis approach from the following publications:
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