Discovery
iD
Time and cost efficient discovery proteomics service!
ID is a standardized discovery proteomics service based on Hyper Reaction Monitoring (HRM-MS™), a next generation proteomics technology invented at Biognosys for quantification of complete proteomes.
ID is a standardized discovery proteomics service based on Hyper Reaction Monitoring (HRM-MS™), a next generation proteomics technology invented at Biognosys for quantification of complete proteomes. HRM enables reproducible and precise quantification of thousands of proteins in a single sample with data independent acquisition (DIA).
The ID proteomic service features a proven HRM workflow with a deep proteome map (spectral library) and a fast turnaround time of 2 to 4 weeks. It is ideal for researchers working on medium to large scale projects that are looking for a reliable and quick service to quantify the proteome changes in their samples.
Post-translational modification analysis or other customized services are also available upon request.
Discovery proteomics services can be carried out for virtually any species and any biological matrix. Detailed information for the most frequently requested sample types:
| Cell culture | Blood | Tissue | Urine | CSF | |
|---|---|---|---|---|---|
| Sample preparation protocols available | yes | yes | yes | yes | yes |
| # of proteins typically identified in sample | 4000-6000 | 300-600 | 4000-6000 | 400-600 | 400-600 |
| Sample amount required | 105-106 cells | 5 ul | 5-10 mg | 100-200 ul | 50-100 ul |
| PTM analysis available | Phosphorylation Glycosylation Acetylation Custom |
Phosphorylation Glycosylation Acetylation Custom |
Phosphorylation Glycosylation Acetylation Custom |
n.a. | n.a. |
Learn more about the revolutionary next-generation proteomics - Hyper Reaction Monitoring (HRM) in our technology section.
Learn about Biognosys tools that enable you to transfer the HRM technology to your own lab.
Project management
Biognosys is committed to providing the best possible results to the customer. We consider confidentiality, pro-active communication and structured project management as key factors in a successful customer relationship. Service projects are usually structured in the following way:
- Project Set-up
- We make sure we understand your research objective
- We determine the most suitable proteomics strategy together with you
- A scientific project manager prepares a project proposal –you take a go/no go decision
- Project Execution
- We start the project the moment we receive the samples from you
- The scientific project manager reports on progress to you in regular intervals
- Project Reporting
- A final report with all data and a description of all methods is delivered upon project completion
- Depending on the number of samples the turnaround time from receiving the samples to final report is 2-4 weeks
Deliverables
The results are presented and discussed with you in a seminar or webinar and results are delivered electronically in PDF and Excel formats and contain:
- Executive summary
- Materials and methods (suitable for publication)
- Complete data matrix:
a) Normalized relative protein intensities for all detected proteins or absolute quantities if stable isotope standards were used.
b) Protein annotation (based on Uniprot Knowledgebase)
- Differential expression analysis:
a) Regulated proteins with regulation factor and p-value.
b) Raw data traces of target proteins available upon request (extracted ion chromatograms).
c) Selected quality control parameters
