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Workflow Kits

Sample Preparation Kit

Simple-to-use standardized sample preparation kit for reproducible mass spectrometry proteomics

The protocols work well with all mass spectrometry based proteomics applications such as SRM/MRM, HRM/DIA/SWATH and shotgun MS/MS.

Product number: Ki-3010

USD

300.00

In stock

Variations in sample preparation between experiments and individual samples are an enormous source of experimental bias in proteomics, especially in high-throughput applications. Since the proteomes of natural samples are extremely complex with typically 100'000s of peptides present, spanning over several orders of magnitude, it is challenging to establish a reliable method for preparing protein samples for analysis by mass spectrometry.

Biognosys’ Sample Preparation Kit is a simple-to-use standardized kit for reproducible mass spectrometry proteomics of mammalian cell cultures and plasma/serum samples. The protocols work well with all mass spectrometry based proteomics applications such as SRM/MRM, HRM/DIA/SWATH and shotgun MS/MS.

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Sample Preparation Kit components

  • Alkylation Solution (1x 10 ml tube)
  • Reduction Stock Solution (1x 0.5 ml tube)
  • LC Solution (1x 10 ml tube)
  • 10x Dilution Buffer (1x 10 ml tube)
  • Denature Buffer (1x 10 ml tube)

The kit components are sufficient for 50 plasma or 50 mammalian cell culture sample preparations to be made at once. It requires 10 µl per sample of plasma/serum or mammalian cell culture pellets with at least 100 µg of total protein per sample. 

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Visit Science Hub to access all scientific and technical content (publications, videos, application notes, posters and more) related to Biognosys technology, products and services.

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What is the purpose of the sample preparation kit and sample preparation kit pro?

Reproducible sample preparation is the key in proteomics to achieve reliable data. The sample preparation kit and the sample preparation kit pro are all-in-one sample preparation kits for mass spectrometry proteomics. The protocols work well with all mass spectrometry based proteomics applications such as SRM/MRM/PRM, HRM/DIA/SWATH and shotgun MS/MS. Biognosys’ Sample Preparation Kit is a simple-to-use standardized kit for reproducible mass spectrometry proteomics of mammalian cell cultures and plasma/serum samples. The Sample Preparation Kit Pro enables the high-throughput processing of 96 cell culture or plasma samples for all mass spectrometry proteomics measurements (SRM/MRM, PRM, HRM, and shotgun).

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