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Service

PlasmaDive

Sample measurement service using a pre-configured assay panel for 100 human plasma proteins!

PlasmaDive is a standardized targeted proteomics service based on Multiple or Parallel Reaction Monitoring (MRM and PRM). MRM and PRM are targeted proteomics techniques that offer highly specific and sensitive quantification of selected proteins from complex biological samples.
 

PlasmaDive is a standardized targeted proteomics service based on Multiple or Parallel Reaction Monitoring (MRM and PRM). MRM and PRM are targeted proteomics techniques that offer highly specific and sensitive quantification of selected proteins from complex biological samples.

The PlasmaDive service includes sample preparation, LC-MS measurements and data analysis of your samples in our state-of-the-art proteomics lab with stable isotope peptides for absolute quantification of 100 plasma proteins (download the full protein list here). PlasmaDive is also available as a ready-to-use kit for your in-house facility. PlasmaDive allows a high-throughput quantification of 100 proteins and is ideal for researchers interested in absolute quantification of the plasma proteins in hundreds or thousands of samples.

Mimimum required volume for PlasmaDive analysis is 10 ul per plasma sample.
 

Project management

Biognosys is committed to providing the best possible results to the customer. We consider confidentiality, pro-active communication and structured project management as key factors in a successful customer relationship. Service projects are usually structured in the following way:

  1. Project Set-up
  • We make sure we understand your research objective
  • We determine the most suitable proteomics strategy together with you
  • A scientific project manager prepares a project proposal – you take a go/no go decision
  1. Project Execution
  • We start the project the moment we receive the samples from you
  • The scientific project manager reports on progress to you in regular intervals
  1. Project Reporting
  • A final report with all data and a description of all methods is delivered upon project completion
  • Depending on the number of samples the turnaround time from receiving the samples to final report is 2-4 weeks

 

Deliverables

The results are presented and discussed with you in a seminar or webinar and results are delivered electronically in PDF and Excel formats and contain:

  • Executive summary
  • Materials and methods (suitable for publication)
  • Complete data matrix:
    a)
    Normalized relative protein intensities for all detected proteins or absolute quantities if stable isotope standards were used.
    b)
    Protein annotation (based on Uniprot Knowledgebase)
  • Differential expression analysis:
    a)
    Regulated proteins with regulation factor and p-value.
    b) Raw data traces of target proteins available upon request (extracted ion chromatograms).
    c) Selected quality control parameters

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