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Multiplexed Assay Panels

PlasmaDeepDive™

Absolute quantification of 100 human plasma proteins in a single measurement!

Multiplexed Assay Panel for absolute quantification of 100 proteins in depleted human plasma/serum.

Available for 48 or 96 samples and includes a license for the SpectroDive™ software
 

PlasmaDeepDive™ 48

Product number: Ki-3006-48

USD

6,750.00

In stock

PlasmaDeepDive™ 96

Product number: Ki-3006-96

USD

12,000.00

In stock

PlasmaDeepDive™ Multiplexed Assay Panel enables absolute quantification of 100 human plasma proteins in a single measurement, covering a dynamic range of 6 orders of magnitude with high precision and reproducibility. It is specifically designed to monitor protein levels in depleted plasma or serum and sets a new standard for clinical research, biomarker screening and development of companion diagnostics.

PlasmaDeepDive™ Multiplexed Assay Panel combines 100 peptide assays in one multiplexed, scheduled MRM or PRM method. It includes 16 peptides corresponding to the typically depleted proteins to monitor the depletion quality, 9 housekeeping proteins for normalization and consequently high reproducibility of the results, and 75 proteins that represent relevant targets for biomarker screening.

PlasmaDive™ Panel Kit contains

  • Reagents and optimized protocol for reproducible sample preparation
  • Stable isotope standards for accurate quantitation
  • iRT Standard  for transfer to virtually any LC setup
  • MS Method for optimized measurements
  • Either 6 or 12 months license for the SpectroDive™ software

 

List of Proteins

Entry Name
UniPot ID
Description

ADIPO_HUMAN

Q15848

Adiponectin

ALS_HUMAN

P35858

Insulin-like growth factor-binding complex acid labile subunit

BDNF_HUMAN

P23560

Brain-derived neurotrophic factor

C1RL_HUMAN

Q9NZP8

Complement C1r subcomponent-like protein

CAD13_HUMAN

P55290

Cadherin-13

CADH5_HUMAN

P33151

Cadherin-5 (CD144)

CBPB2_HUMAN

Q96IY4

Carboxypeptidase B2

CD14_HUMAN

P08571

Monocyte differentiation antigen CD14

CH3L1_HUMAN

P36222

Chitinase-3-like protein 1

CHL1_HUMAN

O00533

Neural cell adhesion molecule L1-like protein

CNDP1_HUMAN

Q96KN2

Beta-Ala-His dipeptidase

CNTF_HUMAN

P26441

Ciliary neurotrophic factor

CO4B_HUMAN

P0C0L5

Complement C4-B

CRAC1_HUMAN

Q9NQ79

Cartilage acidic protein 1

CRF_HUMAN

P06850

Corticoliberin

CXCL7_HUMAN

P02775

Platelet basic protein

CYTC_HUMAN

P01034

Cystatin-C

DIAC_HUMAN

Q01459

Di-N-acetylchitobiase

DOPO_HUMAN

P09172

Dopamine beta-hydroxylase

EGFR_HUMAN

P00533

Epidermal growth factor receptor

ENOG_HUMAN

P09104

Gamma-enolase

FA11_HUMAN

P03951

Coagulation factor XI

FBLN3_HUMAN

Q12805

EGF-containing fibulin-like extracellular matrix protein 1

GFAP_HUMAN

P14136

Glial fibrillary acidic protein

GP1BA_HUMAN

P07359

Platelet glycoprotein Ib alpha chain

GPV_HUMAN

P40197

Platelet glycoprotein V (CD42d)

GRP75_HUMAN

P38646

Stress-70 protein, mitochondrial

GRP78_HUMAN

P11021

78 kDa glucose-regulated protein

HGFA_HUMAN

Q04756

Hepatocyte growth factor activator

HS12A_HUMAN

O43301

Heat shock 70 kDa protein 12A

HS71L_HUMAN

P34931

Heat shock 70 kDa protein 1-like

HS74L_HUMAN

O95757

Heat shock 70 kDa protein 4L

HSP71_HUMAN

P08107

Heat shock 70 kDa protein 1A/1B

HSP74_HUMAN

P34932

Heat shock 70 kDa protein 4

HSP76_HUMAN

P17066

Heat shock 70 kDa protein 6

HSP7C_HUMAN

P11142

Heat shock cognate 71 kDa protein

HSP7E_HUMAN

Q0VDF9

Heat shock 70 kDa protein 14

IBP2_HUMAN

P18065

Insulin-like growth factor-binding protein 2

IBP3_HUMAN

P17936

Insulin-like growth factor-binding protein 3

IDHC_HUMAN

O75874

Isocitrate dehydrogenase [NADP] cytoplasmic

IGF1_HUMAN

P05019

Insulin-like growth factor I

IGF2_HUMAN

P01344

Insulin-like growth factor II

IL10_HUMAN

P22301

Interleukin-10

IL1AP_HUMAN

Q9NPH3

Interleukin-1 receptor accessory protein

ITIH3_HUMAN

Q06033

Inter-alpha-trypsin inhibitor heavy chain H3

LBP_HUMAN

P18428

Lipopolysaccharide-binding protein

LDHB_HUMAN

P07195

L-lactate dehydrogenase B chain

LEP_HUMAN

P41159

Leptin

LUM_HUMAN

P51884

Lumican

LYAM1_HUMAN

P14151

L-selectin (CD62L)

MGMT_HUMAN

P16455

Methylated-DNA--protein-cysteine methyltransferase

MINP1_HUMAN

Q9UNW1

Multiple inositol polyphosphate phosphatase 1

MMP9_HUMAN

P14780

Matrix metalloproteinase-9

MMRN1_HUMAN

Q13201

Multimerin-1

NCAM1_HUMAN

P13591

Neural cell adhesion molecule 1 (CD56)

NEBU_HUMAN

P20929

Nebulin

NEST_HUMAN

P48681

Nestin

NEUT_HUMAN

P30990

Neurotensin/neuromedin N

NGF_HUMAN

P01138

Beta-nerve growth factor

NMB_HUMAN

P08949

Neuromedin-B

NPY_HUMAN

P01303

Pro-neuropeptide Y

NRP1_HUMAN

O14786

Neuropilin-1 (CD304)

P53_HUMAN

P04637

Cellular tumor antigen p53

PHLD_HUMAN

P80108

Phosphatidylinositol-glycan-specific phospholipase D

PROZ_HUMAN

P22891

Vitamin K-dependent protein Z

PTEN_HUMAN

P60484

Phosphatase and tensin homolog (PTEN)

PZP_HUMAN

P20742

Pregnancy zone protein

RETN_HUMAN

Q9HD89

Resistin

SAMP_HUMAN

P02743

Serum amyloid P-component

SYPL1_HUMAN

Q16563

Synaptophysin-like protein 1

TIMP1_HUMAN

P01033

Metalloproteinase inhibitor 1

TKN1_HUMAN

P20366

Protachykinin-1

TSP1_HUMAN

P07996

Thrombospondin-1

VASN_HUMAN

Q6EMK4

Vasorin

VWF_HUMAN

P04275

von Willebrand factor

A1AT_HUMAN

P01009

Alpha-1-antitrypsin

A2MG_HUMAN

P01023

Alpha-2-macroglobulin

ALBU_HUMAN

P02768

Serum albumin

APOA1_HUMAN

P02647

Apolipoprotein A-I

APOA2_HUMAN

P02652

Apolipoprotein A-II

CO3_HUMAN

P01024

Complement C3

FIBA_HUMAN

P02671

Fibrinogen alpha chain

FIBB_HUMAN

P02675

Fibrinogen beta chain

FIBG_HUMAN

P02679

Fibrinogen gamma chain

HPT_HUMAN

P00738

Haptoglobin

IGHA1_HUMAN

P01876

Ig alpha-1 chain C region

IGHG1_HUMAN

P01857

Ig gamma-1 chain C region

IGHG2_HUMAN

P01859

Ig gamma-2 chain C region

IGHM_HUMAN

P01871

Ig mu chain C region

TRFE_HUMAN

P02787

Serotransferrin (Transferrin)

TTHY_HUMAN

P02766

Transthyretin

A1BG_HUMAN

P04217

Alpha-1B-glycoprotein

CERU_HUMAN

P00450

Ceruloplasmin

HEMO_HUMAN

P02790

Hemopexin

IC1_HUMAN

P05155

Plasma protease C1 inhibitor

ITIH2_HUMAN

P19823

Inter-alpha-trypsin inhibitor heavy chain H2

ITIH4_HUMAN

Q14624

Inter-alpha-trypsin inhibitor heavy chain H4

KNG1_HUMAN

P01042

Kininogen-1

VTDB_HUMAN

P02774

Vitamin D-binding protein

VTNC_HUMAN

P04004

Vitronectin

Please contact us for support.

PlasmaDeepDive™ Multiplexed Assay Panel Kit contains reagents, plastic ware, optimized protocol for reproducible sample preparation, stable isotope standards with integrated iRT peptides for accurate quantitation, and Panel Plug-in for method set-up and data analysis with SpectroDive™ Software.

PlasmaDeepDive™  48 Multiplexed Panel Kit components

  • Reference Peptide Mix (1x 1.1 ml glass vial)
  • Alkylation Solution (1x 10 ml tube)
  • Reduction Stock Solution (1x 0.5 ml tube)
  • LC Solution (1x 10 ml tube)
  • 10x Dilution Buffer (1x 10 ml tube)
  • Denature Buffer (1x 10 ml tube)
  • Dissolution Buffer (1x 2.0 ml tube)
  • 96-well MACROSpin Plate (1x with 2x 96-well plate included)
  • 96-well Plate (3x packaged separately)
  • 96-well Plate Sealer (6x)
  • PlasmaDeepDive™ MRM or PRM Panel Plug-in for SpectroDive software
  • PlasmaDeepDive™ Multiplexed Assay Panel Manual
  • 6 months license for the SpectroDive™ software 

 

PlasmaDeepDive™  96 Multiplexed Panel Kit components

  • Reference Peptide Mix (2x 1.1 ml glass vial)
  • Alkylation Solution (2x 10 ml tube)
  • Reduction Stock Solution (2x 0.5 ml tube)
  • LC Solution (2x 10 ml tube)
  • 10x Dilution Buffer (2x 10 ml tube)
  • Denature Buffer (2x 10 ml tube)
  • Dissolution Buffer (2x 2.0 ml tube)
  • 96-well MACROSpin Plate (1x with 2x 96-well plate included)
  • 96-well Plate (3x packaged separately)
  • 96-well Plate Sealer (6x)
  • PlasmaDeepDive™ MRM or PRM Panel Plug-in for SpectroDive software
  • PlasmaDeepDive™ Multiplexed Assay Panel Manual
  • 12 months license for the SpectroDive™ software

 

Supported instruments

MRM

  • Thermo Scientific™ TSQ™ Series (Vantage, Quantum, Quantiva)
  • AB SCIEX API 4000™
  • AB SCIEX Triple Quad™ 5500 
  • Agilent 6400 Series
 PRM
  • Thermo Scientific™ Q Exactive™ Series
  • Thermo Scientific™ Orbitrap Fusion™ Series

 

If your instrument is not on the list please contact us to get detailed information on the instruments we support.

The kit is sufficient for analysis of 96 human depleted plasma or serum samples and allows independent processing of 2 batches of 48 samples each. PlasmaDeepDive™ Multiplexed Assay Panel requires as little as 50 µg of protein from depleted plasma sample and 48 hours to provide actionable clinically-relevant data. The panel is perfect for large-scale experiments requiring high-throughput.

PlasmaDeepDive™ Multiplexed Panel Panel is also offered as a Service for measurements in Biognosys facility.

Please contact us for support.

Visit Science Hub to access all scientific and technical content (publications, videos, application notes, posters and more) related to Biognosys technology, products and services.

Peer-reviewed papers

  1. Kiseleva OI, Romashova YA, Moskaleva NE, Petushkova NA, Teryaeva NB, Belyaev AY, Lisitsa AV. Plasma preparation to measure FDA-approved protein markers by selected reaction monitoring. Clin Transl Med. 2015; 4(1):32.
If we forgot to include your publication that uses our technology, please don’t hesitate to let us know at info@biognosys.com.

Please contact us for support.

What is the difference between PlasmaDive, PlasmaDiveMini and PlasmaDeepDive?

PlasmaDive and PlasmaDiveMini monitor an identical set of 100 proteins levels in human plasma or serum samples with high throughput. Each stable isotope standard peptide represents a human plasma protein, with almost half of the panel’s proteins being considered a putative cancer, cardio-vascular or metabolic biomarker. The difference is only in the number of samples that can be processed. PlasmaDive is made for 96 samples and PlasmaDiveMini is made for 20 samples.

PlasmaDeepDive™ multiplexed assay panel targets 100 proteins to be measured in one scheduled MRM or PRM experiment. It includes 16 peptides corresponding to the typically depleted proteins in human plasma analysis, which can be used to monitor the depletion quality, 9 housekeeping proteins for normalization and consequently high reproducibility of the results, and 75 proteins that represent relevant targets for biomarker screening. 

Can I retrieve the protein identities used in the kits?

Yes, all proteins covered by the respective kits can be found in our online shop, under the support material section of each product. Additional information can be retrieved from the Science Hub section of the Biognosys website. 

What is the dynamic range of PlasmaDive and PlasmaDeepDive combined?

Together the two kits span 6-7 orders of magnitude dynamic range with high precision and reproducibility. Additional information on the targeted proteins and use cases can be found in Science Hub section of the Biognosys website. 

Can I measure only a subset of the proteins in each kit?

Of course, assays of only a subset of the proteins in one kit can be generated and measured. Thereby the measurement time can be reduced and the sensitivity of the analysis increased.

Can I use the PlasmaDeepDive peptides with native (not depleted) plasma?

Yes, this is possible. However, sample preparation protocol needs to be updated and some of the analytes in PlasmaDeepDive are low abundant proteins though and might not be detectable in non-depleted plasma in all conditions. Please contact us for additional information.

Please contact us for support.

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