Phosphorylation of proteins is central in a variety of functional states of proteins. It is a predominant post-translational modification involved in signal transduction involving cascades of phosphorylation events of kinases. Understanding phosphorylation has been essential in accelerating the understanding of health and disease, while protein kinases have been a major interest in the treatment of cancer and chronic inflammatory diseases.

Biognosys’ has developed its discovery proteomics (HRM-MS™) technology to enable the quantification of thousands of phospho-proteins and phospho-site.

The Phospho Profiling service features a set of proven HRM workflows offering a choice of depth for the proteome profiling and a fast turnaround time of 5-6 weeks for standard projects. The workflow is applicable for small and large-scale studies with thousands of samples. It is ideal for researchers who are looking for a deep phospho-proteome quantification service to better understand cellular activation in a hypothesis-free manner.

The Phospho Profiling services can be carried out for virtually any species and biological matrix.

Learn more about the revolutionary next-generation proteomics - Hyper Reaction Monitoring (HRM™) in our technology section.

Learn about Biognosys tools that enable you to transfer the HRM technology to your own lab. 

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Project Management

Biognosys is committed to providing the best possible results to our customers. We consider confidentiality, proactive communication, and structured project management as key factors in a successful customer relationship. Service projects are usually structured in the following way:

1. Project Set-up

• We make sure we understand your research objective
• We determine the most suitable proteomics strategy together with you
• Our scientific team prepares a detailed project proposal – you may accept or decline the contract

2. Project Execution

• A scientific project manager is assigned to your project
• We initiate the project the moment we receive your samples
• You receive regular reports on the progress of your project

3. Project Reporting

• A final report with data analysis and a description of all methods is delivered upon project completion. Depending on the number of samples, the turnaround time from receiving the samples to the final report is 5-6 weeks

Deliverables

The results are presented and discussed with you in a seminar or webinar and delivered electronically in PDF and Excel formats that contain:

• Executive Summary
• Materials and Methods (suitable for publication)
• Complete Data Matrix:
  a) Normalized relative phospho-peptide intensities for all detected phospho-sites/phospho-proteins 
  b) Protein annotation (based on Uniprot Knowledgebase)

• Data Interpretation:
  a) Differentially regulated proteins with regulation factor and p-value
  b) Coefficient of variation analysis
  c) Principal component analysis
  d) Partial least square discriminant analysis
  e) Clustered heat map
  f) Pathway analysis
  g) Custom analysis upon request

Please contact us for support.