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Contract Research

Mechanism of Action Studies

Discovery proteomics service for deep proteome quantification.

The Mechanism of Action Studies is a standardized discovery proteomics service based on Hyper Reaction Monitoring (HRM-MS™), a next-generation proteomics technology invented at Biognosys for quantification of complete proteomes.


 

The Mechanism of Action service is used to identify the specific biochemical interactions in which a substance (e.g. a drug candidate) produces a pharmacological effect. Biognosys’ discovery proteomics platform provides a broader and deeper understanding of the molecular targets and the relevant biological pathways that are responsive to compound treatment by quantifying thousands of proteins among control and treatment samples for subsequent correlation analysis. 

The Mechanism of Action service features a set of proven HRM workflows offering a choice of depth for the proteome profile and a fast turnaround time of 5-6 weeks for standard projects. It is ideal for researchers who are looking for a deep proteome quantification service to identify the mechanism of action and characterize molecular pathways in a hypothesis-free manner.

Post-translational modification analysis and other customized services are also available upon request.

The Mechanism of Action service can be carried out for virtually any species and biological matrix.

 

Detailed information for the most frequently requested sample types:

 

  Tissue Cell-lines Plasma/Serum Urine CSF
Sample preparation protocols available yes yes yes yes yes
# of proteins typically identified in the sample 3’000-10’000 3’000-10’000 300-600 (depleted
plasma 500-1’400)
600-2’600 500-1’500
Sample amount required 5-10 mg 105-106 cells 5-10 ul 100-200 ul 50-100 ul

 

Learn more about the revolutionary next-generation proteomics - Hyper Reaction Monitoring (HRM™) in our technology section.

Learn about Biognosys tools that enable you to transfer the HRM technology to your own lab. 

 

Request quote

 

Project Management

Biognosys is committed to providing the best possible results to our customers. We consider confidentiality, proactive communication, and structured project management as key factors in a successful customer relationship. Service projects are usually structured in the following way:

1. Project Set-up

  • We make sure we understand your research objective
  • We determine the most suitable proteomics strategy together with you
  • Our scientific team prepares a detailed project proposal – you may accept or decline the contract

2. Project Execution

  • A scientific project manager is assigned to your project
  • We initiate the project the moment we receive your samples
  • You receive regular reports on the progress of your project

3. Project Reporting

  • A final report with data analysis and a description of all methods is delivered upon project completion. Depending on the number of samples, the turnaround time from receiving the samples to the final report is 5-6 weeks

 

Deliverables

The results are presented and discussed with you in a seminar or webinar and delivered electronically in PDF and Excel formats that contain:

 

  • Executive Summary
  • Materials and Methods (suitable for publication)
  • Complete Data Matrix:
    a)
    Normalized relative protein intensities for all detected proteins or absolute quantities if stable isotope standards were used 
    b)
    Protein annotation (based on Uniprot Knowledgebase)
  • Data Interpretation:
    a) Differentially regulated proteins with regulation factor and p-value
    bCoefficient of variation analysis
    cPrincipal component analysis
    dPartial least square discriminant analysis
    eClustered heat map
    fPathway analysis
    gCustom analysis upon request

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