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Discovery

HighD

Discovery proteomics service with maximum proteome coverage!

HighD is a discovery proteomics service optimized for maximum depth of analysis and highest number of identified proteins. It is based on Hyper Reaction Monitoring (HRM-MS™), a next generation proteomics technology invented at Biognosys for quantification of complete proteomes.
 

HighD is a discovery proteomics service optimized for maximum depth of analysis and highest number of identified proteins. It is based on Hyper Reaction Monitoring (HRM-MS™), a next generation proteomics technology invented at Biognosys for quantification of complete proteomes. HRM enables reproducible and precise quantification of thousands of proteins in a single sample with data independent acquisition (DIA).

The HighD proteomic service features an ultra-long LC gradient and is performed on the latest high-resolution mass spectrometer instruments. It is ideal for researchers working on small to medium sized projects that are looking for the deepest proteome coverage on the market.

Post-translational modification analysis or other customized services are also available upon request.

Discovery proteomics services can be carried out for virtually any species and any biological matrix. Detailed information for the most frequently requested sample types:

 

 

  Cell culture Blood Tissue Urine CSF
Sample preparation protocols available yes yes yes yes yes
# of proteins typically identified in sample 6000-8000 500-800 6000-8000 700-1200 600-900
Sample amount required 105-106 cells 5 ul 5-10 mg 100-200 ul 50-100 ul
PTM analysis available Phosphorylation
Glycosylation
Acetylation
Custom
Phosphorylation
Glycosylation
Acetylation
Custom
Phosphorylation
Glycosylation
Acetylation
Custom
n.a. n.a.

 

Learn more about the revolutionary next-generation proteomics - Hyper Reaction Monitoring (HRM) in our technology section.

Learn about Biognosys tools that enable you to transfer the HRM technology to your own lab.

 

Project management

Biognosys is committed to providing the best possible results to the customer. We consider confidentiality, pro-active communication and structured project management as key factors in a successful customer relationship. Service projects are usually structured in the following way:

  1. Project Set-up
  • We make sure we understand your research objective
  • We determine the most suitable proteomics strategy together with you
  • A scientific project manager prepares a project proposal –you take a go/no go decision
  1. Project Execution
  • We start the project the moment we receive the samples from you
  • The scientific project manager reports on progress to you in regular intervals
  1. Project Reporting
  • A final report with all data and a description of all methods is delivered upon project completion
  • Depending on the number of samples the turnaround time from receiving the samples to final report is 3-5 weeks

 

Deliverables

The results are presented and discussed with you in a seminar or webinar and results are delivered electronically in PDF and Excel formats and contain:

  • Executive summary
  • Materials and methods (suitable for publication)
  • Complete data matrix:
    a)
    Normalized relative protein intensities for all detected proteins or absolute quantities if stable isotope standards were used.
    b)
    Protein annotation (based on Uniprot Knowledgebase)
  • Differential expression analysis:
    a)
    Regulated proteins with regulation factor and p-value.
    b) Raw data traces of target proteins available upon request (extracted ion chromatograms).
    c) Selected quality control parameters

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