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Service

Absolute Quant

Customized proteomics service for absolute quantification of selected proteins!

Absolute Quant is a customized targeted proteomics service based on Multiple or Parallel Reaction Monitoring (MRM and PRM). MRM and PRM are targeted proteomics techniques that offer highly specific and sensitive quantification of selected proteins from complex biological samples.
 

Absolute Quant is a customized targeted proteomics service based on Multiple or Parallel Reaction Monitoring (MRM and PRM). MRM and PRM are targeted proteomics techniques that offer highly specific and sensitive quantification of selected proteins from complex biological samples.

The Absolute Quant service includes sample preparation and assay development with stable isotope peptides for absolute quantification of the selected proteins. The LC-MS measurements of your samples can be performed in our state-of-the-art proteomics lab or the method can be transferred to your facility. Absolute Quant allows a high-throughput quantification of up to 150 proteins and is ideal for researchers interested in absolute quantification of the selected proteins in hundreds or thousands of samples.

Absolute Quant analysis is available for virtually any species and any biological matrix. Detailed information for the most frequently requested sample types:

 

  Cell culture Blood Tissue Urine CSF
Sample preparation protocols available yes yes yes yes yes
Sample amount required 105-106 cells 5 ul 5-10 mg 100-200 ul 50-100 ul

 

Learn more about the targeted proteomic methods in our technology section.

Learn about Biognosys tools that enable you to implement the targeted proteomics technology to your own lab.

 

Project management

Biognosys is committed to providing the best possible results to the customer. We consider confidentiality, pro-active communication and structured project management as key factors in a successful customer relationship. Service projects are usually structured in the following way:

  1. Project Set-up
  • We make sure we understand your research objective
  • We determine the most suitable proteomics strategy together with you
  • A scientific project manager prepares a project proposal – you take a go/no go decision
  1. Project Execution
  • We start the project the moment we receive the samples from you
  • The scientific project manager reports on progress to you in regular intervals
  1. Project Reporting
  • A final report with all data and a description of all methods is delivered upon project completion
  • Depending on the number of samples the turnaround time from receiving the samples to final report is 2-4 weeks

 

Deliverables

The results are presented and discussed with you in a seminar or webinar and results are delivered electronically in PDF and Excel formats and contain:

  • Executive summary
  • Materials and methods (suitable for publication)
  • Complete data matrix:
    a)
    Normalized relative protein intensities for all detected proteins or absolute quantities if stable isotope standards were used.
    b)
    Protein annotation (based on Uniprot Knowledgebase)
  • Differential expression analysis:
    a)
    Regulated proteins with regulation factor and p-value.
    b) Raw data traces of target proteins available upon request (extracted ion chromatograms).
    c) Selected quality control parameters

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