Spectronaut® 19 goes above and beyond to bring you more biologically relevant targets than before, and solutions that make large-scale proteomics analysis a breeze. This new version doesn’t disappoint when it comes to identifications either with improved quantification and proteome depth.
“Spectronaut 19 offers significant leaps in terms of quantification, proteome depth, and scalability. Under the hood, we have made substantial progress with the AI models integrated into Spectronaut 19 which results in an increased ability to detect differentially abundant peptides and proteins.”
Lukas Reiter, PhD, CTO of Biognosys
Spectronaut continues to offer record-breaking protein identification rates. Compared to Spectronaut 18, version 19 provides a 10% average increase in protein identifications, a 14% increase in precursor identifications as well as a 10% and 16% increase in identifications with CV <20%, for protein groups and precursors, respectively.
You can be confident that you get the best protein coverage with Spectronaut across different instruments, gradient lengths, sample types, and complexity.
The goal of most DIA experiments is to discover differentially expressed analytes. To benchmark this, we spiked ‘A. thaliana’ and ‘C. elegans’ into ‘H. Sapiens’ background in varying amounts across 10 different conditions. The CQE was repeated with three different gradients on Bruker’s timsTOF HT .
With Spectronaut 19 every analysis provided more biologically relevant targets across different gradient lengths and fold changes, whether big or small. This makes experiments more actionable due to a more sensitive discovery of candidates.
Spectronaut 19 introduces extended support for plexDIA workflows with channel-specific condition setup and channel-based differential analysis as well as a new option for channel-level FRD control.
Analysis with Spectronaut 19 improved identifications for channel IDs and protein groups for 3-plex dimethyl labeled samples (M. Thielert et.al., Mol Syst Biol 2023) and 3-plex mTRAQ (J. Derks et al., Nature Biotechnology 2023).
This new version of Spectronaut also features novel support for the analysis of diagonal dia-PASEF and expands support for PTM workflows by introducing normalization by input functions and PTM stoichiometry.
With Spectronaut 19 you now get significantly improved RAM consumption that enables you to streamline your large-scale experiments.
This is one of many improvements Spectronaut 19 offers to address the challenges of modern large-scale proteomics analysis.
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