prm-PASEF HT: High sensitivity and throughput for absolute quantitation of plasma proteins

The fusion of trapped ion mobility spectrometry (TIMS) with parallel reaction monitoring (PRM), particularly through the prm-PASEF ® technique, is indeed a groundbreaking development in proteomics. This innovative approach leverages the parallel accumulation-serial fragmentation (PASEF) mode to synchronize ion release from TIMS with selective precursor isolation, reducing the noise in the peptide ion spectra without sacrificing sensitivity.