Reduced analysis time and more identified targets with Spectronaut 7

In a recently published application note Thermo Scientific concludes that the high throughput capabilities of Biognosys’ Spectronaut 7 lead to “remarkable reduction in total analysis time and substantially higher number of identified peptide targets”.

Combining data independent acquisition (DIA) techniques with their new Orbitrap Fusion Lumos, Thermo Scientific carried out a complete protein quantification of whole cell lysates on two non-small lung cancer cell lines, one which shows drug resistance. More than 1200 significantly regulated protein groups were identified, many belonging to important signaling pathways, including the MAPK and mTOR pathways.

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