The paper by Karlsson et al is the first published genome-wide MRM assay repository for a single organism. Using group A Streptococcus as a model organisms, the authors demonstrate the design, construction, and testing of over 10’000 MRM assays for all 1905 proteins found in the group A Streptococcus genome. The paper outlines how MRM assays can be constructed using the patent protected method of synthetic peptide libraries and Biognosys’ iRT kit at a large scale and how the MRM assays were subsequently used to outline the spatial distribution of the group A Streptococcus proteome.
Karlsson C, Malmström L, Aebersold R, Malmström J: Proteome-wide selected reaction monitoring assays for the human pathogen Streptococcus pyogenes, Nature Communications 2012, 3, article number 1301.
The publication is free available on Nature Online Library with access to pdf and html version.