We would like to announce today a new revolutionary approach for high-precision prediction of peptide retention time (high-precision iRT) specifically designed for the targeted analysis of data independent acquisition (DIA or SWATH) data sets. The high-precision iRT approach is described in a study recently published in Proteomics by Bruderer et al. entitled “High-precision iRT prediction in the targeted analysis of data-independent acquisition and its impact on identification and quantitation”.
The newly developed high-precision iRT concept extends the set of original 11 iRT peptides to thousands of anchor points derived from the actual sample. These anchor points are stored in Spectronaut, a professional software for DIA or SWATH data analysis, which automatically takes the available peptides in each specific sample to build a high quality spectral library. The high-precision iRT concept results in 15% more precursor IDs in a DIA analysis compared to the original iRT and 25% more precursor IDs in a DIA run when retention time prediction was not used for spectral library generation (Bruderer et al. 2016).