Lidia Wrobel, Sandra M. Hill, Alvin Djajadikerta, Marian Fernandez-Estevez, Cansu Karabiyik, Avraham Ashkenazi, Victoria J. Barratt, Eleanna Stamatakou, Anders Gunnarsson, Timothy Rasmusson, Eric W. Miele, Nigel Beaton, Roland Bruderer, Yuehan Feng, Lukas Reiter, M. Paola Castaldi, Rebecca Jarvis, Keith Tan, Roland W. Bürli & David C. Rubinsztein. Nature Communications

Enhancing the removal of aggregate-prone toxic proteins is a rational therapeutic strategy for a number of neurodegenerative diseases, especially Huntington’s disease and various spinocerebellar ataxias. This study shows how a compound binding to VCP D1 ATPase enhances proteasomal neurotoxic protein clearance. Furthermore, our proprietary High-Resolution Limited Proteolysis (HR-LiP) technology was used to 3D-map the binding site of the compound under investigation for potential use in neurodegenerative diseases. As a result, we could confidently validate the binding site on the target and explain the observed phenotype.

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Presenter: Yuehan Feng

Authors: Marco Tognetti, Christoph Messner, Roland Bruderer, Dominique Kamber, Jacqueline Darrow, Alexandra Lewis, Jakob Vowinckel, Abhay Moghekar, Lukas Reiter, Yuehan Feng
In collaboration with with the Johns Hopkins University School of Medicine

Overview: Since the genome remains invariant throughout the lifespan, it is essential to understand the effects of aging on the proteome, as proteins play an integral role in all cellular functions and mediate the effects of epigenetic alterations. At the SfN Annual Meeting we presented a study in collaboration with Johns Hopkins University School of Medicine on proteomic profiling of biomarker candidates for Alzheimer’s Disease in plasma and CSF with our TrueDiscovery™ platform.

Download the poster to learn more about how the quantitative proteoform map is linked to healthy and pathological aging, providing a rich resource for therapeutic target discovery.

E-Poster presented at HUPO Reconnect 2021
Presenter: Yuehan Feng

Abstract Number: 162
Topic: 01. Brain Rewiring in Neurological Disorders

Presenter: Ino Karemaker
Date: November 5

The poster discusses plasma and CSF data collected with our TrueDiscovery™ platform in collaboration with The Johns Hopkins University School of Medicine in plasma.

Out of the almost 6,000 proteins identified in CSF and more than 3,000 in plasma from young healthy control subjects, patients with mild cognitive impairment, age-matched healthy controls, and Alzheimer’s patients, we were able to generate a comprehensive and quantitative map of proteomes linked to healthy and pathological aging. Download the poster to discover more.

Presenter: Marco Tognetti

Date: Wednesday, June 7
Topic area for your poster: Neurodegenerative Disease Research I

Presenter: Yuehan Feng

Date: Tuesday, Sep 19, 2023, 11:45 AM – 1:00 PM
Session: PP03

Poster presented at ASBMB 2022

Presenter: Yuehan Feng
Wednesday, August 17

While aging remains the most significant risk factor for neurodegenerative diseases (NDs) such as Alzheimer’s disease (AD) or Parkinson’s disease (PD), the biological pathways that are altered in healthy aging vs. pathologic aging leading to neurodegeneration remain to be elucidated. Since the genome remains invariant throughout the lifespan, it is essential to understand the effects of aging on the proteome as proteins play an integral role in all cellular functions and mediate the effects of epigenetic alterations. In addition, while the brain remains protected by the blood-brain barrier, there is increasing evidence that events such as systemic inflammation, metabolic dysfunction significantly affect the risk for the development of various NDs.