Biognosys is proud to release the latest generation of SpectroMine, our software solution for DDA data analysis, at the
HUPO Connect 2020.
SpectroMine 2 brings with major improvements, which offer you unsurpassed performance for DDA proteomics. We prepared two seminars for you, which we would like to share below:
SpectoMine 2 integrates the state-of-the-art Pulsar search engine, now offering full support for ion mobility technologies (e.g., FAIMS and PASEF) and achieving more identifications thanks to deep learning augmentation. In this seminar, you will receive insights into how the software works internally, and how you can easily analyze ILQ data (e.g. TMTpro) with SpectroMine 2.
“Multiplexed Proteomics for Understanding Molecular Biology”
Mikhail Savitski, PhD (Team Leader & Head of Proteomics Facility – EMBL)
An application of multiplexed proteomics for investigating molecular biology will be presented. In particular, examples of how thermal proteome profiling can provide insights into the functional aspects of the proteome will be discussed. The seminar will highlight the benefit of using SpectroMine for these types of experiments.
Erwin Schoof, PhD (Assistant Professor – Technical University of Denmark)
A single-cell proteomics strategy was used to investigate the characteristics of individual cells in acute myeloid leukemia (AML). Taking advantage of the latest advancements in LC-MS instrumentation and data acquisition, the new TMTPro reagents, and SpectroMine 2.0, an unprecedented map of protein expression was built-in individual AML cells. Furthermore, a computational pipeline (SCeptre) was built that effectively processes single-cell proteomics data and permits the extraction of cell-specific proteins. Strong enrichment of stem cell-specific proteins was found in the LSC and progenitor compartments, and protein expression signatures were identified that clearly distinguish cell differentiation stages. This work lays a solid foundation for implementing global single-cell proteomics studies in proteomics labs across the world.
The presentations were approved by the Program Committee as an independent activity held in conjunction with the HUPO Connect 2020. The presentations are not sponsored or endorsed by HUPO Connect 2020.