The Mechanism of Action Studies service identifies the specific biochemical interactions in which a drug candidate produces a pharmacological effect. Actionable insights are achieved through the quantification of thousands of proteins among control and treatment samples for subsequent correlation analysis.
The application is based on Biognosys’ proprietary Hyper Reaction Monitoring (HRM™) technology.
We offer a choice of depth for the proteome profiling. Post-translational modification analysis and other customized services are also available upon request.
The service is ideal for researchers who are looking for a deep proteome quantification service to identify the mechanism of action and characterize molecular pathways in a hypothesis-free manner.
Resources
Case Studies
Deep Proteomic Profiling of CD4+ and CD8+ T Cell-Depleted Tumor Models in PD-1 Treatment
T-cell exhaustion is one of the mechanisms leading to tumor progression. It is especially relevant during immunotherapy with ant-PD-1/PD-L1. In our study with Crownbio Sciences (Sa [...]
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Deliverables
The results are presented and discussed with you in a dedicated conference call and delivered electronically in PDF and Excel format.
Executive Summary
Materials and Methods
(suitable for publication)Complete Data Matrix
a) Normalized relative protein intensities for all detected proteins or absolute quantities if stable isotope standards were used
b) Protein annotation (based on Uniprot Knowledgebase)
Data Interpretation
a) Differentially regulated proteins with regulation factor and p-value
b) Coefficient of variation analysis
c) Multivariate analysis (e.g. PCA)
d) Hierarchical clustering analysis
e) Functional analysis (e.g. GO/Pathway analysis)
f) Custom analysis upon request
Sample types and depths
Mechanism of Action Studies can be carried out for virtually any species and biological matrix.
Project management
Biognosys is committed to providing the best possible results to our customers at the fastest possible project turnaround time.
