Application note on acetaminophen induced proteome changes in liver micro tissues

Today Biognosys released an application note “Proteomic analysis of acetaminophen toxicity in liver micro tissues using data independent acquisition (DIA) on a quadrupole-Orbitrap™ mass spectrometer and Spectronaut™ 9 software”. 

In this study, the DIA based workflow Hyper Reaction Monitoring (HRM) was used for quantitative proteomic analysis of acetaminophen induced changes in liver micro tissues. To maximize the performance of the HRM workflow we have acquired data using Thermo Scientific™ Q Exactive™ HF mass spectrometer and analyzed it with Spectronaut™ software. Downstream analysis of the proteomic changes was performed with fuzzy c-means clustering and pathway enrichment analysis.

In single shot DIA measurements of 12'000 cells per sample we have reproducibly identified and quantified more than 3'000 proteins with median CVs as low as 7.5%. Downstream analysis shows various acetaminophen induced proteome changes already in sub-therapeutic concentrations.

You can download the application note here. To find additional information on the latest advancements in high-content protein analysis visit the Science Hub, a comprehensive library of articles, posters, application notes, webinars and other publications that showcase Biognosys technology, products and services.

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