Phosphorylation of proteins is a central modulatory mechanism for protein function. The signal transduction cascades, which are regulated by phosphatases and kinases, are important targets for the treatment of cancer and chronic inflammatory diseases. These pathways do not act in isolation and only a global view of phosphorylation events can reveal the true mode action of a drug.
The Phospho Profiling application utilizes Biognosys’ proprietary Hyper Reaction Monitoring (HRM™) technology, with a choice of depth for the phospho-proteome profiling.
The service is ideal for researchers who are looking for a deep phospho-proteome quantification to better understand cellular activation in a hypothesis-free manner.
Phospho Profiling can be applied in large-scale studies, quantifying the complete phospho-proteome in thousands of samples with high throughput.
Resources
Case Studies
A High-throughput Platform for Proteome and Phospho-proteome Profiling of Tumor Tissues – Case Study
Precision oncology requires a detailed molecular understanding of tumor biology, which is best characterized by the study of the proteome. Biognosys has developed a high throughput [...]
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Deliverables
The results are presented and discussed with you in a dedicated conference call and delivered electronically in PDF and Excel formats.
Executive Summary
Materials and Methods
(suitable for publication)Complete Data Matrix
a) Normalized relative intensities for all detected phospho-sites, phospho-peptide, and phospho-proteins
b) Protein annotation (based on Uniprot Knowledgebase
Data Interpretation
a) Differentially regulated proteins with regulation factor and p-value
b) Coefficient of variation analysis
d) Hierarchical clustering analysis
e) Functional analysis (e.g. kinase enrichment and/or pathway analysis)
f) Custom analysis upon request
Sample types and depths
Phospho Profiling Studies can be carried out on samples from any species and are most commonly applied on cell lines or tissue.
Project management
Biognosys is committed to providing the best possible results to our customers at the fastest possible project turnaround time.
